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...is interesting because by changing certain amino acids you can change the specificity,” explained Zhanar...consecutive base in its RNA target. By changing amino acids, it’s possible to produce a protein that binds an...and improve, Abil says they will make those new plasmids available through Addgene, and they hope others...

...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

....1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging...10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. A protein-....2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...

....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from... LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/gkl1108...

...thanks for depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use...encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher...

...tools (and can have secondary functions as detection aids.) When a single protein is expressed, with the intent...antibodies, resins, beads, and other purification aids readily available. Choosing the best tag for your...Antibody protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags...

...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...

...steps into this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology...these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...Resources on the Addgene Blog Check out other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find... Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene ...Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing...Search for a plasmid Find Addgene's most popular plasmids ...

...transduce? So why use a virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ...translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by the ribosome. ...

....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

...whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins...spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV absorbance...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that... Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

...fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein...