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We narrowed to 879 results for: ids

Showing: 401 - 420 of 879 results

  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...is interesting because by changing certain amino acids you can change the specificity,” explained Zhanar...consecutive base in its RNA target. By changing amino acids, it’s possible to produce a protein that binds an...and improve, Abil says they will make those new plasmids available through Addgene, and they hope others...

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ....1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging...10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. A protein-....2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...

  6. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...tools (and can have secondary functions as detection aids.) When a single protein is expressed, with the intent...antibodies, resins, beads, and other purification aids readily available. Choosing the best tag for your...Antibody protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags...

  8. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...steps into this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology...these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

  10. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...transduce? So why use a virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

  11. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...Resources on the Addgene Blog Check out other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find... Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

  15. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

  16. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

  18. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that... Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

  19. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins...spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV absorbance...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...
Showing: 401 - 420 of 879 results