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We narrowed to 877 results for: ids

Showing: 401 - 420 of 877 results

  2. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...tools (and can have secondary functions as detection aids.) When a single protein is expressed, with the intent...antibodies, resins, beads, and other purification aids readily available. Choosing the best tag for your...Antibody protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags...

  3. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...steps into this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology...these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

  4. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

  5. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...Resources on the Addgene Blog Check out other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find... Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

  6. Viral Vectors 101: Transductions

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    Blog Post
    ...transduce? So why use a virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

  7. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

  8. Meet the New Addgene Board Members!

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    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post
    ....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  12. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...their fast adoption. However, in many cases the plasmids themselves are only the starting point for the...that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research...

  14. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins...spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV absorbance...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

  16. K. phaffii: Rising to the Occasion

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    Blog Post
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

  17. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post
    ...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that... Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

  18. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post
    ...gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among ...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...

  19. Using AAV for Neuronal Tracing

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    Blog Post
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

  20. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...this blog post for tips on using BLAST to verify plasmids. Analyzer guide A quick guide to all of ...browse the repository and quickly identify the plasmids and features you need for your next experiment...
Showing: 401 - 420 of 877 results