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Showing: 401 - 420 of 884 results
  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests ...14%). Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests...effects fairly well. However, we observed one complication in the China data – there is a period of 1 month...fundamental scientific discovery and high-impact applications.       Alvin Hsu is a graduate student at Harvard...
  2. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...the peer-review system of journals and grant applications, for example. However, at the same time, it'.... So, young researchers need to publish good publications in a short period of time and may need to do...available. Visit Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts...
  3. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...especially valuable for regulatory approval and public acceptance. Recently, viral vectors have been successfully...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...elaborate protocols are available in our earlier publications (Karmakar et al., 2024; Karmakar et al., 2025...
  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...have real conversations with other scientists. Click Here to Visit Joanne’s AMA I've heard of Reddit,...Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...
  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...Institute, Public Domain, National Institutes of Health, Wikipedia user Aimecie~commonswiki, Public Domain...let alone the human cost. Here are just a few publications which have since been retracted due to cell ...profiles are available for all the major cell lines on public databases. Image attributions (from top to bottom...
  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box...give people more work. Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...
  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ..., mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human...
  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...
  9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...How can one ensure that an FP fusion with a non-cytosolic protein functions properly? We suggest starting... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...
  10. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells...chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...
  11. E11 Bio PRISM Collection

    Type
    Collection
    ...components can be used separately for additional applications. Park & Sheridan et al. (2025) use plasmids ...circuit mapping and reconstruction. Potential applications could also include using individual or subsets...
  12. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...cell types, and research applications. Labeling neurons with Brainbow The Lichtman group developed the original... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...
  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...
  14. Antibody Production

    Type
    Collection
    ...cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...
  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...al. 2025 under a CC-BY-NC-ND 4.0 International license. While the GENIE team tested and deposited...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....DNA vector that contains everything needed to replicate inside a host cell but with space to insert your...bioRxiv, under a CC-BY-NC-ND 4.0 International license. These plasmids are part of the BRAIN Initiative...
  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...equipment on a lab bench. The best part? Less complicated math. Bioengineering is a field that has been...methods is that they all follow the same general cyclical process (Figure 2). The differences typically ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...
  17. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review of neuroscience...SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene modification technology ...Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology... J. Simon, et al. "Silencing neurons: tools, applications, and experimental constraints." Neuron 95.3 ...
  18. Promega Plasmid Collection

    Type
    Collection
    ...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...
  19. AAV Packaged on Request

    Type
    Collection
    ...remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal... using the PHP.eB Caltech serotype in future publications, please acknowledge Viviana Gradinaru and cite...
Showing: 401 - 420 of 884 results