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We narrowed to 876 results for: lic

Showing: 401 - 420 of 876 results

  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...early I’m fortunate to live in a town with a good public school system that has made strong efforts in the...simplistic and I would insult the students. Too complicated and I would quickly lose their interest. I won...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....examples I used in my presentation had only become public in the previous two weeks, which demonstrated to...

  2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... and have been adapted for a wide variety of applications - as mutagenesis tools for genetic studies (... The process also creates a 5-bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family...at one or multiple desired genomic loci. Many applications of these systems have already been demonstrated..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

  3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...the peer-review system of journals and grant applications, for example. However, at the same time, it'.... So, young researchers need to publish good publications in a short period of time and may need to do...available. Visit Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts...

  4. Promega Plasmid Collection

    Type
    Collection
    ...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...

  5. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...especially valuable for regulatory approval and public acceptance. Recently, viral vectors have been successfully...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...elaborate protocols are available in our earlier publications (Karmakar et al., 2024; Karmakar et al., 2025...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box...give people more work. Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ..., mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human...

  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...have real conversations with other scientists. Click Here to Visit Joanne’s AMA I've heard of Reddit,...Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    ...Institute, Public Domain, National Institutes of Health, Wikipedia user Aimecie~commonswiki, Public Domain...let alone the human cost. Here are just a few publications which have since been retracted due to cell ...profiles are available for all the major cell lines on public databases. Image attributions (from top to bottom...

  11. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...How can one ensure that an FP fusion with a non-cytosolic protein functions properly? We suggest starting... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells...chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

  13. Arf GTPase Family

    Type
    Collection
    ...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

  15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...cell types, and research applications. Labeling neurons with Brainbow The Lichtman group developed the original... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...al. 2025 under a CC-BY-NC-ND 4.0 International license. While the GENIE team tested and deposited...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....DNA vector that contains everything needed to replicate inside a host cell but with space to insert your...bioRxiv, under a CC-BY-NC-ND 4.0 International license. These plasmids are part of the BRAIN Initiative...

  17. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...equipment on a lab bench. The best part? Less complicated math. Bioengineering is a field that has been...methods is that they all follow the same general cyclical process (Figure 2). The differences typically ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

  18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review of neuroscience...SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene modification technology ...Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology... J. Simon, et al. "Silencing neurons: tools, applications, and experimental constraints." Neuron 95.3 ...

  19. Ras Pathway

    Type
    Collection
    ...-25% of all human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene... Research Return to top Ras Pathway - Gene List Click on a name to find available plasmids for the gene...

  20. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place...
Showing: 401 - 420 of 876 results