We narrowed to 474 results for: mag
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TypeBlog Post...). Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under CC-BY ...
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Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...applicable to those positions you have already held. Imagine this situation: You’ve spent hours scouring a company... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... in a single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has,... -
CRISPR Antimicrobials
TypeBlog Post... resensitization, or may also cause cell death. Image from Beisel et al., licensed under a Creative Commons... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...within a plate that can hold up to 96 tubes (see image below). Each plate has numbers and letters to give... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...hundreds of plasmid requests out each day. As you may imagine, it keeps all of us quite busy. Though there still... -
Plasmids 101: Protein tags
TypeBlog Post... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... Host killing (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a... -
Plasmids 101: Codon usage bias
TypeBlog Post...transcription factor binding sites) As you might imagine, it’s not easy for humans to balance all of these... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...enthusiasts to start a microscopy seminar to connect the imaging community and to create a platform for discussions... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...popular Chinese social media platforms. As you can imagine, shipping to so many countries means that our shipping... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post.... Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...optimization of fluorescent proteins, and live cell imaging approaches. Erik is also an avid long distance ... -
Lab to Office Culture Shock
TypeBlog Post...coat! These are the things the rest of the world imagines when they think “scientist.” But leaving the bench... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post... Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal of Neuroscience... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020. Broad tropism: ... -
Viral Production at Addgene
TypeBlog Post...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced... -
How to Be an Excellent Trainee
TypeBlog Post...experiments. Unlike in lab courses, where everything is magically made up by helpful TAs or assistants, and sitting...