We narrowed to 478 results for: mag
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TypeBlog Post...applicable to those positions you have already held. Imagine this situation: You’ve spent hours scouring a company...
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Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...outshines FRET — BiFC experiments only require that you image and measure the fluorescence of a single FP. Your... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...more immediate and dire than Zimmer could have imagined. Does this detract from the quality of the book... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...rolling mean gave us a crude insight into the magnitude of the effect COVID-19 had on our data. Because... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...which is likely a career path none of them had imagined for a scientist. I think that establishing this... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...-1 locus. Figure 1: Schematic of enCHIP. Image from Fujita and Fuji, 2013. Prior to the development... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...work for all bacteria; may cause plasmid/host DNA damage. Choosing an antibiotic resistance Choosing the... -
An Introduction to Adenovirus
TypeBlog Post...). Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015 under CC-BY ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... in a single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has,... -
CRISPR Antimicrobials
TypeBlog Post... resensitization, or may also cause cell death. Image from Beisel et al., licensed under a Creative Commons... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...within a plate that can hold up to 96 tubes (see image below). Each plate has numbers and letters to give... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...hundreds of plasmid requests out each day. As you may imagine, it keeps all of us quite busy. Though there still... -
Plasmids 101: Protein tags
TypeBlog Post... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses... -
Plasmids 101: Codon usage bias
TypeBlog Post...transcription factor binding sites) As you might imagine, it’s not easy for humans to balance all of these... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...enthusiasts to start a microscopy seminar to connect the imaging community and to create a platform for discussions... -
Career Insights: Technical Support Specialist
TypeBlog Post...Many thanks to Eric Perkins and Stephanie Hays for images used in this post. Klaus Wanisch is an Outreach... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...PMID: 9618448 Diaz R, Rech J, Bouet J-Y (2015) Imaging centromere-based incompatibilities: Insights into... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Singleton. Figure 1: The Binning Singletons logo. Image: James et al., 2020. Help me stranger - Finding...