We narrowed to 565 results for: mme

1. Communicating Your Science With Help From ComSciCon

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   Blog Post

   ... it's not always recognized and valued for its immense importance, it may well be what determines whether...

2. Antibodies 101: Flow Cytometry Controls

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   ... appropriate isotype controls linked on their commercial antibody pages. Flow cytometry can be a complicated...

3. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...percentage of the proteins inside a cell have a commercial antibody against them. There’s also a question...

4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...thoughts about this technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex...

5. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   ... you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring group ...

6. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   Blog Post

   ...and make sure to choose one that comes highly recommended from someone you trust. A good coach can make...

7. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   Blog Post

   ...the DNA-binding domain is modifiable and can be programmed to bind to essentially any DNA sequence desired...

8. Antibodies 101: Secondary Antibodies

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   ...blot can be run in a single day, I really cannot recommend it except in the most dire of circumstances.) ...

9. What's New in CRISPR - November 2021

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   ...Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f nuclease...

10. Antibodies 101: Fab Fragments

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    Blog Post

    ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...

11. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...monomer in the cell type tested. To conclude, we recommend that you try a couple of different fluorescent...

12. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...protein as the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and...

13. Plasmids 101: The protein expression toolbox

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    ...References References Nabet et al. The dTAG system for immediate and target-specific protein degradation. Nature...

14. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...Lucas, Burstein, David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

15. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ... al., 2011).  Additionally, researchers have reprogrammed regenerating tissues without using genome engineering...

16. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are...

17. Human Germline Editing Using CRISPR

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    Blog Post

    ...probably seen this study (and various articles and commentaries) pop up on Twitter and Facebook, shared by biologists...

18. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...downstream steps. It’s also possible to determine immediate effects of signaling vs the delayed effects because...

19. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

20. The Future of Research Symposium Boston 2015

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    Blog Post

    ...hired to produce results, not scientists”  These comments are well correlated with published data. For example...