We narrowed to 568 results for: mme

1. Multiplex Genome Editing with CRISPR-Cpf1

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   ...thoughts about Cpf1 multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   ...before a study is conducted. Editors and reviewers recommend changes, and if everyone agrees that the research...

3. Antibodies 101: Flow Cytometry Controls

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   ... appropriate isotype controls linked on their commercial antibody pages. Flow cytometry can be a complicated...

4. Antibodies 101: Monoclonal Antibodies

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   ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   ...daughter. It’s super fun and we are enjoying that immensely. Paratore: I have two children active in sports...

6. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...percentage of the proteins inside a cell have a commercial antibody against them. There’s also a question...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...thoughts about this technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex...

8. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ... you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring group ...

9. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   ...and make sure to choose one that comes highly recommended from someone you trust. A good coach can make...

10. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    ...the DNA-binding domain is modifiable and can be programmed to bind to essentially any DNA sequence desired...

11. Antibodies 101: Fab Fragments

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    ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...

12. Plasmids 101: The protein expression toolbox

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    ...References References Nabet et al. The dTAG system for immediate and target-specific protein degradation. Nature...

13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...protein as the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and...

14. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...Lucas, Burstein, David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

15. Antibodies 101: Secondary Antibodies

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    ...blot can be run in a single day, I really cannot recommend it except in the most dire of circumstances.) ...

16. What's New in CRISPR - November 2021

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    ...Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f nuclease...

17. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ... al., 2011).  Additionally, researchers have reprogrammed regenerating tissues without using genome engineering...

18. Troubleshooting Your Plasmid Cloning Experiment

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    ...major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are...

19. Human Germline Editing Using CRISPR

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    ...probably seen this study (and various articles and commentaries) pop up on Twitter and Facebook, shared by biologists...

20. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...downstream steps. It’s also possible to determine immediate effects of signaling vs the delayed effects because...