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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 902 results for: nes

Showing: 401 - 420 of 902 results

  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested...means researchers who use VSV-G can deliver their genes of interest to a variety of cells and study their...Skimming the list, one gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used... of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the...

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...societal implications even before the discovery of genes themselves.  How has scientific progress in genetic...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ...that results in gene knockouts.   Star Trek: Into Darkness (2013) which is the remake of the space-melodrama...Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus ...

  3. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified...Zhang lab has tested in a variety of mammalian cell lines. Cas9, of course, is the key component of most CRISPR...gene that made it less effective in certain cell lines. This point mutation has been corrected in V2.0....gRNA of interest in a variety of mammalian cell lines. Alternative forms of this vector contain GFP-tagged...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...overview of the instrumentation A flow cytometer combines three systems to analyze single cells from a mixture...degree of internal complexity such as extensive membranes produces a greater SSC.  Protein expression, measured...targets, measured by fixing and permeabilizing membranes For intracellular protein targets, users must ...propidium iodide, can only enter cells with disrupted membranes as is the case with dead or dying cells. DNA binding...the cells, amine reactive dyes interact with free amines in the cytoplasm causing dead cells to fluoresce...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution, ...region   The constant region of the heavy chain determines the antibody’s isotype - that is, one of five...which come from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their...antibody allows the protein to be detected by chemiluminescence or fluorescence. Western blots are frequently...Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier...

  6. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...screens using CRISPR technology, gRNAs to knockout genes – are essential screening components, the model ...Sometimes, you might have a few positive control genes on which to test the validity of your biological... a new assay or both, and you have no idea what genes are involved. Or you don’t know how well your assay...definition, the kinome is a well-annotated collection of genes that can provide a useful starting point for developing...

  7. Editor's Choice, August 2016

    Type
    Blog Post
    ...Directed Mutagenesis by PCR Google Forums Round Up: First Impressions of NgAgo CRISPR Between the Genes: How...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...technology may make some uses of Site Directed Mutagenesis by PCR obsolete! Honorable mentions Of course...

  8. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace...lab culture. The aim of these meetings is to give honest, constructive feedback, set tangible research and...personal development through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve...person organize his or her thoughts to facilitate honest conversation. Goals and Planning Worksheet: ...

  9. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ... more often than other disciplines. The majority of tweets for most disciplines contain scientific domains...#8. Figure 1: Highly Represented Scientific Disciplines on Twitter. Twitter users were identified as ...Follower networks of subgroups of scientific disciplines: social (left), life (center), and physical (...

  10. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce irreversible...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts yielded... from the “S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together... chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this purpose?...

  11. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...into the following categories: Over 300 empty backbones with 100+ distinct fluorophores to choose from...constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins “It...It’s a great collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working...collection with such variety.” In most cases, backbones are available to attach the gene of interest to...

  13. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV, these two genes are...which contains the AAV structural and packaging genes, the adenoviral helper plasmid which contains the...Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products...

  14. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...currently houses over 890 CRISPR screens in 629 cell lines, each manually curated and annotated from more than...and power of CRISPR-based library screens in cell lines from humans and other species. By accurately compiling...browse through screens performed in relevant cell lines or conditions. This increased ease of access not...screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. Each screen curated...

  15. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary ...cannot survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most... that!    References and resources References Clynes RA, Towers TL, Presta LG, Ravetch JV. Inhibitory...

  16. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...Giving honest and helpful feedback gets easier with practice so you must build it into your routines. This...their work.  Check out Joanne's Reddit AMA   Guidelines for giving feedback Use formal and informal ...differently? Beware of indirect language You must be honest about performance problems. While giving corrective...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... Encodes insert(s) A plasmid with one or more genes, protein coding sequences, or other elements inserted...designed for cloning a gene/insert into it. Empty backbones often contain epitope tags or fusion proteins ...Insert *Note: This page will not appear for empty backbones Please enter the name of any gene contained within...Addgene ID in the "Backbone Manufacturer" box for backbones requested through Addgene. For example, you could...Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I hereby certify that I have disclosed all known...

  18. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...raw plasmid sequence and display frequently used genes and control elements. Development of this tool required...feature sequences due to genetic drift and the use of genes from different microbial strains. It proved to be...sequence feature that may be used to make fusion genes, detection needs to occur even if one or two codons... our database, or if you see an opportunity to harness common feature detection in a new way, please contact...

  19. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Kamens several years ago through the Healthcare Businesswomen’s Association and it was through this connection... systematically test the function of all 20,000 genes in disease models and then focus drug discovery ...early age, I was fascinated by the impact that medicines can have on human health. As a teenager in high...the field of gene therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate...

  20. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...pooled library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage ...
Showing: 401 - 420 of 902 results