Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 985 results for: nin

Showing: 401 - 420 of 985 results

  1. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...detection had over 1,000 authors. The chapter on deep learning addresses the dilemma of creating a technology... “The most obvious future consequence of the undermining of the scientific process and the marginalization... the summer of 2020 that the consequences of undermining science are even more immediate and dire than...himself has written entire books about optogenetics. Defining science and summarizing its past, present, and...task to set oneself. As a scientist who enjoys learning a little bit about many different things (part...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    ...plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning Read... use a phenol chloroform extraction to remove remaining proteins and RNase. Finally, DNA is precipitated...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids..., as detected by qPCR, but this did not reduce cloning efficiencies.  When compared to fresh columns, ...

  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...discretionary money. They also usually have a good event-planning base and will know how to reserve space, time ...themselves as “scientists.” We had some accountants running our math tables!  Be realistic about what you...you can teach  5-10 year olds in a single evening. Grades K-5 cover a broad range of attention spans, skill... simple. I honed the complexity of the talk by running it by the teachers and sharing some slides on Twitter...Even the most ambitious teachers, who work hard maintaining a current, dynamic curriculum, would be hard-...

  4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... direct RNA-guided transposition. By natively combining the DNA integration capabilities of transposases...with Type I-F INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and...s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of CRISPR-Cas systems by ...Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F (2019) RNA-guided DNA insertion with...science.aax9181 Strecker J, Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-...

  5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...HypaCas9, are great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species....critical enzymatic residues has been converted to an alanine (D10A or H840A), changing its double-strand cut...available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA, a reverse....   Ok, I have a Cas9! What do i do next? Designing or selecting a sgRNA Addgene may carry a validated...expression systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...identifies nine enemies that stand in the way of creative thinking. I won’t go through all nine here, but...found that many researchers feel they lack formal training in a variety of transferable skills. At Addgene...absolutely a transferable skill developed during PhD training. Creativity is required to develop new projects...’re stuck drawing stick figures, remember your training as a scientist has been building your creativity...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... genome. These off-target sequences may be unintentionally mutated along with the desired target locus...sequences when used in place of wild-type SpCas9. The remaining variants (VQR, EQR, and VRER) recognize novel ...Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni...Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo genome editing...Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (2015) Cpf1 Is a Single RNA-Guided ...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...checkpoint.  The plasmid is self-transmissible, meaning that the plasmid is conjugative and carries the...inducibility (Toh et al. 2023), and additional cloning sites (Keen 1988).  Find broad host range recombinant... Origin of the Broad Host Range Plasmid RK2: Positioning of Various Motifs is Critical for Initiation ...1988). Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene, 70(1), 191–197...R. (1988). Chapter 15—Broad-Host-Range Plasmid Cloning Vectors for Gram-Negative Bacteria. In R. L. Rodriguez...

  9. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    ... shift to remote learning due to COVID-19. We had many protocols for plasmid cloning and working with ...details about personal protective equipment to learning about how to use a tissue culture hood and other...on our Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists!...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...been around since 1975 thanks to the Nobel prize winning work of Milstein and Köhler who developed hybridoma...by infecting bacteria for additional rounds of panning and scFv characterization.    Figure 2: ...by infecting bacteria for additional rounds of screening. Created with Biorender.com.   Some phage ...and VH sequences. Phage display also allows for screening scFv generated from the B cell antibody genes ...Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute to the disulfide...

  11. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...months. Jennifer and I spent about an hour that morning scrambling. Everything we had set up involved multiple...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...smooth filming in the future, but we were finally refining the process down to a place I could be happy with...

  12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...sj.emboj.7600385 McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing shRNA...shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing the plasmid backbone... and just needs to be taken into account when designing experiments.   {% module_block module "widget_...Kilikevicius, A., Meister, G., & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...3’ flaps containing the edited sequence anneal with each other, while the 5’ flaps containing the original...First, twin prime editing installs two sequences containing attB/P recombinase landing sites on opposite ...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome at this ...rat brain sections stained with Anti-GFAP. The staining patterns from recombinant antibodies produced ...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...around 20 minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome ....  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl (Ambystoma...community.  The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research development...out the GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in ...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

  15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...involves the introduction of a transgene construct containing fluorescent proteins (XFP) of different colors...not undergo recombination can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice...are that it does not use ubiquitous promoters, meaning that not all cell types can be labeled, and that...labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express the... a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous...

  16. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...iBiology which hosts an amazing amount of useful, entertaining science lab, career, and policy content. Watch...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many...force it. You can always relate to scientists by explaining how your work fits in the bigger science picture...that this might sound condescending, consider explaining the term on a slide but not reading the explanation...practice. I always take a poll of scientists in training to see how many in my audiences have given a 50...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial host strain...The pCRT7 NT TOPO vector contains a mutant pSer-containing GFP that can be used as a positive control during... researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule that binds specifically...that wasn’t originally a serine (e.g. mutating a threonine residue to a phosphoserine) to control for effects...large proteins by Western blotting using Phos-tag containing gel." Nat. Protoc. doi 10 (2008). Journal. Resources...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...Off-target effects” a vague catch-all term for unintended consequences in an experiment. For CRISPR work...In other words, they can sometimes localize to unintended sites with sequence similarity to the on-target...on-target site. When a CRISPR-Cas system localizes to unintended sites and performs its programmed function there...not Homologous Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023...factor, etc.) this control is usually not very meaningful. Unfortunately, there really aren’t any effective...

  19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded DNA repair templates Short...electroporation methods using in mouse transgenics. Designing an Easi-CRISPR knock-in allele To design a knock-in...make the first two bases of the left homology arm guanine (i.e. GG); this design has been shown to increase..., you can add a silent PAM or guide mutation. Designing an Easi-CRISPR conditional allele The design for... can amplify the desired region using primers containing the homology arms on both sides and the T7 promoter...

  20. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... aim of improving professional development and training in a globalized world where individuals can exchange...IMP 2014-15, University of Valencia): “At the beginning, I registered at IMP to have an opportunity to...throughout the entire year. In addition to their direct training role as mentors to Spanish mentees, mentors also...addition to a pre-program self-assessment survey defining expectations of the program and their baseline...resources, and entrepreneurial visions thereby opening new frontiers for young talent around the world...
Showing: 401 - 420 of 985 results