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  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency...observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can...leverage this system in new, creative ways. Today’s post will focus on one such strategy--the FLEx switch...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...IgG2a? Will my choice affect my experiment? In this post we will review some of the main factors that affect...using polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

  7. 6 Tips for Grant Writing

    Type
    Blog Post
    ...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ...was a PhD student at University College Dublin & a post-doc at Cambridge University where he studied mechanisms...published in a paper, but positive outcomes can include poster presentations, talks about your work, or even the...

  8. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models...

  9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...Half (113) expressed in retinal cells four weeks post-injection and 14% had targeted expression in specific...technologies Browse all of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find...mouse retinas were injected into macaques and postmortem human retinas. About 25% of these AAV synthetic...Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez,...

  10. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First... the Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...2016). We quickly assembled a team of students, postdocs and professors to move the project forward as ...

  11. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...Taylor-Parker contributed to the writing of this post. Additional Resources on the Addgene Blog Visit...

  14. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...This post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...”, it is certainly the most robust. In this blog post, we will discuss how CRISPR libraries are being ...
Showing: 401 - 420 of 783 results