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...experiment are known as your "panel". In this blog post, we'll discuss the principles and process of designing...resolve this issue. Make sure to check out our blog post on color compensation to learn more about them. ...bd-spectrum-viewer.   Conclusion You made it! In this post, you learned about the principles behind fluorophores...

...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University. She studies... Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future of Research...chair of the postdoctoral engagement committee and active member of the university's Postdoctoral Association...in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical School working...runs hand in hand with policy. Alexandra is a postdoctoral researcher in the University of Michigan Department...continue the dialogue. Erin Golden is currently a postdoctoral fellow at the University of Colorado Anschutz...

...This guest post was contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids...progress.Learn more about cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods...

...also work equally well in dividing and quiescent, post-mitotic mammalian cells. Applications of CRISPR...Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...

...into play? We’ll dive into those questions in this post as we explore… the life and times of the myc tag...substance P has an amide group added to its C-terminus post-translationally, which turned out to be critical...

...their interaction. You can read our previous blog post for more on BiFC principles and experimental design...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...

...This post was contributed by Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We...resources on the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs...

...Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...second chance” mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence...1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 ...

...make the most of these pages, we've written this post to guide you through them and make the best use ...publications which have used the plasmid (see our blog post) for more information about this feature!).  This...

...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other ...

... Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational killing by the hok/sok system of plasmid...to efficient cloning Read all Plasmids 101 blog posts Read about knockout/knock-in plasmids Resources...

...experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and...account for batch-to-batch variability. Check out this post to learn more about different ways to titer AAV....

...Additional Resources on the Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene...for large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources...

...acid purification methods not mentioned in today’s post? Let us know in the comments! Additional resources...protocols and tips Browse our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular...

... blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts Resources...

...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...the Addgene blog Browse all of our CRISPR blog posts Check out other CRISPR methods for bacteria Resources...

...please sign up here.  Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools...from Chertkova et al., 2020. In their BioRxiv post, the authors generated and described several improved...

...simple procedure is often taken for granted. In this post, we take a moment to review what exactly you are...one of the most popular options, so most of this post is based on reading results from a NanoDrop. The...

...libraries can be quite challenging to use! In this post, I’ll walk you through how to prep and transduce...perform your pooled library screen…but that’s a blog post for another day.  Using pooled libraries can be ...

...applications from across disciplines now available. In this post, we’ll review the inspiration behind ExM, how it...samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should start...