We narrowed to 610 results for: sam

1. Donations from Addgene to Yield Answers for Rare Disease Researchers

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   Blog Post

   ...we are working on several genetic diseases at the same time. The limitation of budget restrained us from...

2. Another Pathway into Cells: iTOP

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   ... better than immortalized lines derived from the same cell type; however, their usage has been limited...

3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   ...The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter to...

4. What Good Citizenship Can Do for Reproducibility in Science

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   ... won’t work. Even if it does, they don’t get the same result. So, then they end up investing time that...

5. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki’s lab engineered SpCas9 (SpCas9-NG) with ...

6. Visualizing Genomic Loci with CRISPR-Sirius

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   ...combination of MS2, PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from...

7. Improving Transduction Efficiency with Exosome AAVs

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   ... have a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells...

8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   ...robot that will “swim” in the river, take water samples at specific locations, measure fluorescent signals...

9. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   ...exposure times (5 minutes vs 10 seconds) to achieve the same level of killing as Mg-2I-dL5**. Mg-2I-dL5** also...

10. Visualizing Translation at the Single Molecule Level

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    ...highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA complexes...

11. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post

    ...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting...

12. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    Blog Post

    ...this mutant fly to one lacking the mutation in the same genetic background. In other cases, YFG or one of...

13. The MTA at Addgene

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    ...developments.  MTAs also mean that everyone is on the same page as to how the shared reagents should be used...

14. The Importance of High Titer for AAV Transductions

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    ...possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles to ...

15. Early Career Researcher Toolbox: Free Tools for Science Writing

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    ...and Linux.  Mendeley and Zotero have a lot of the same features, but this chart highlights the differences...

16. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    ...researchers find other publications that have used the same plasmid through the “Articles Citing this Plasmid...

17. How to Lead a Great Meeting

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    Blog Post

    ...will usually suffice to keep both parties on the same page. Managers will get a sense of the employee’...

18. Management for Scientists: Delegating is Key

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    ...documentation helps make sure everyone is on the same page. Effective delegation allows you to make the...

19. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...be imaged together, you will need to consider the same factors as when choosing an individual fluorescent...

20. Advice for Scientists Starting a Lab

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    Blog Post

    ...useless.” How do you run your lab differently or the same as your adviser did? Tom Ellis merges the influence...