We narrowed to 734 results for: son
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TypeBlog Post...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson...
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Antibodies 101: Affinity Reagents
TypeBlog Post...entirely new affinity reagent. Fig. 1: A comparison of an antibody (left) to several alternative ... Rachel Leeson... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...interest and its localization in the membrane. The lesson we learned from these studies is that the tag protein...Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (1988) Purification of ... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you take time... Rachel Leeson... -
The time and cost required to make a plasmid
TypeBlog Post...written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies... -
Developing Lab Management Software for Biology
TypeBlog Post...cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the... -
Working with Nuclear Receptors
TypeBlog Post...making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...gene and cell-based therapies in the new era of personalized medicine (2017). 1st Gen AdVs are stripped...gene and cell-based therapies in the new era of personalized medicine." Genes & diseases 4.2 (2017): 43-63... -
Rabies and Neuronal Tracing
TypeBlog Post...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body’... 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our... -
Hot Plasmids - October 2020
TypeBlog Post...and inhibitory synaptic proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal...library from Addgene New from the viral service By Jason Nasse We regularly add new viral aliquots from our... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...of data together indicate that 35-pieces is a reasonable upper limit for Golden Gate Assembly size. Ultimately... Rachel Leeson... -
Viral Vectors 101: Chemogenetics
TypeBlog Post... Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023. References and Resources References...Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation. New from the viral service By Jason Nasse We regularly add new viral aliquots from our... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...antibodies recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). ... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...20463753. PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in... -
Tips for Technical Support Calls
TypeBlog Post...! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet searches are done via...potentially faulty product. Please remember that the person at the other end of that email you’re writing is... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...and so should be handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element...