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We narrowed to 772 results for: LIS

Showing: 421 - 440 of 772 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...up clear tasks for each member of the team to accomplish so that many elements of the project were able...Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought...

  4. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

  5. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for...PCR is that it can amplify DNA using only a short list of reagents and several heating and cooling steps...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...not map to the reference sequence. The resulting list of hits is then carefully reviewed. As mentioned...

  7. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...not better, than antibodies for all of the above listed molecular techniques, but they can also be expressed...

  11. MXS Chaining

    Type
    Blog Post
    ...The tradeoffs between various cloning methods are listed in Table 2. For example,  chaining-based cloning...used in various chaining-based cloning methods are listed in Table 3. The goal of high throughput cloning...

  12. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... to reflect upon before they attempt to make essentialist connections between biology and ancestral groups...the long and ugly history of reporting false essentialist links between biology and ancestry group as ...

  14. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

  15. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK...

  19. Lab to Office Culture Shock

    Type
    Blog Post
    ...polling some Addgene colleagues, we’ve come up with a list of things to consider as you make this transition...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ...
Showing: 421 - 440 of 772 results