We narrowed to 778 results for: Dos

...right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important roles in...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

...electrical charges propagated through multiple cells to do things like translate perceived signals to physical...

...Integrative and conjugative elements (ICEs): what they do and how they work." Annual review of genetics 49 ...

...CRISPR-Cas system they block can be found here. How do anti-CRISPR proteins work? Anti-CRISPRs proteins ...

... used the library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition...

...ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, ...

... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

...how to design primers for cloning purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM...36.8 μL Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix...added to the master mix. Forward and Reverse Primers DO NOT get added to a master mix. Place reaction tubes...reverse primers to the thinned walled PCR tubes. Note: Do this before adding the master mix so you know that...according to the length of the target sequence. FAQ How do I design primers? See our reference page on how to...The ideal setup for this troubleshooting step is to do one reaction with each, and one reaction with both...templates. What does each ingredient specifically do? Template DNA: Contains the portion of DNA that we...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...

... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

...was actually engineered to target the CNS. How well do these AAVs transduce the CNS and PNS? Both plasmids...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral...blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail are injected, by chance lots...the two green neurons near the arrows. When lower doses of virus are used in order to decrease the number...AAV.PHP.eB capsid. Both viruses were IV injected at a dose of 1x1011 vg per mouse and the level of transduction...AAV.PHP.S capsid. Both viruses were IV injected at a dose of 1x1012 vg per mouse and the level of transduction...-off transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

...in the biosynthetic pathway. Why plant scientists do transient leaf transformations The development of...

...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...