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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 819 results for: cell

Showing: 631 - 660 of 819 results

  1. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...examples could be an endogenous sample of wild type cells, purified protein, or a peptide. The positive control... target antigen, such as a knockout or knockdown cell line or tissue samples where the target is not expressed...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...identifying new genes required for stem and progenitor cell maintenance in planarians in Developmental Biology...junction complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology... his teaching style, with the former offering excellent training in improvisation and the latter making...

  3. How to Write a Scientific Review Article

    Type
    Blog Post
    ...a review article on histone modifers in B and T cells with my adviser when I was a graduate student. Naturally...acetyltransferases and histone deacetylases in B-and T-cell development, physiology and malignancy." Genes &...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ...incubated at stationary phase for too long, the cells in your culture will no longer be viable. The time...graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage and has...

  6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system will...US, I very much enjoyed interactions with many excellent colleagues at NYU and visiting scientists, such...In addition, I've been very lucky to work with excellent colleagues in my lab. On the other hand, I feel...

  7. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...useful for both in vitro and in vivo work with many cell types, as CAG is a ubiquitous and constitutive promoter...

  8. Editor's Choice, July 2016

    Type
    Blog Post
    ...Studies Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...2016 Click on the links below to read all of our excellent posts from July: Addgene at the ASM Microbe 2016...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...

  10. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...

  12. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...co-founder of Addgene. Melina received her Ph.D. in Cell Biology from Harvard University, where she studied...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

  13. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh...developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher...biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ... that your reference genes are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle...reference genes to save on plate space. For many cell lines and tissue types, appropriate reference genes...at determining the best options for a particular cell or tissue type (such as here, here, or here).  If...your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want ...

  21. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

  22. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...

  25. Distribution to Industry

    Type
    Collection
    ...plasmids for detecting GPCR activity in a variety of cell types. Protein Purification Optimization Kit Using...

  27. Technical Design of a Western Blot

    Type
    Blog Post
    ... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient.  ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins...

  29. Microbiology Resources

    Type
    Collection
    ...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...

  30. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...and the resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if...
Showing: 631 - 660 of 819 results