...CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system will...US, I very much enjoyed interactions with many excellent colleagues at NYU and visiting scientists, such...In addition, I've been very lucky to work with excellent colleagues in my lab. On the other hand, I feel...

...useful for both in vitro and in vivo work with many cell types, as CAG is a ubiquitous and constitutive promoter...

...Studies Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...2016 Click on the links below to read all of our excellent posts from July: Addgene at the ASM Microbe 2016...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...

... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...

...Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Oct;23(10):1163-71. doi: 10.1038/cr.2013.122...

...institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh...developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher...biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for...

...co-founder of Addgene. Melina received her Ph.D. in Cell Biology from Harvard University, where she studied...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...

... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...

...occurs when bacterial cells regulate gene expression in response to changes in cell density. At the mixer...

...particles that can transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type...

... sure that the world will go on without my whole-cell extract from 2009. Join a newsletter to stay up-...

... that your reference genes are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle...reference genes to save on plate space. For many cell lines and tissue types, appropriate reference genes...at determining the best options for a particular cell or tissue type (such as here, here, or here).  If...your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want ...

...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...

...is immediately ready to transform into competent cells. The master mix of enzymes can be purchased from...mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically ...

...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology and scientific...

...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511...

... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient.  ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins...

...Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016/j.molcel.2015.04.025...

...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...

...and the resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if...

...GCaMP6f-P2A-nls-dTomato Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox...

... chemically induce neuronal activity in specific cell types. See our Chemogenetics Plasmids and Chemogenetics...

...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

...tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific...

...limit of detection for arg1 expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal...

...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...