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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: REN

Showing: 841 - 880 of 1018 results

  1. Advice for Choosing a Research Project

    Type
    Blog Post
    ...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

  2. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

  6. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...

  9. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...

  10. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

  11. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22...

  12. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

  14. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...

  15. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...

  19. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule so you can rely ...Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential publications...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

  22. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...

  23. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...the interview process was several short talks by current faculty members and it just blew me away….  I have...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...

  24. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...

  25. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  26. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ...

  27. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

  28. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

  29. Hot Plasmids - May 2022

    Type
    Blog Post
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

  30. Antibodies 101: ChIP

    Type
    Blog Post
    ...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

  31. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be...

  32. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania...

  33. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

  34. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant...

  35. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering...

  37. Addgene: The First Twenty Years

    Type
    Blog Post
    ...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...

  38. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what...

  40. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...
Showing: 841 - 880 of 1018 results