We narrowed to 903 results for: nes

...maintaining the quality of reagents, especially shared ones.  The importance of QC Plasmids are a fundamental...

...exceptions to our standard workflow.  Table 1: Guidelines for the data fields in the deposit spreadsheet...

...orientation, and type of target sites that flox your genes of interest, a powerful FLEx switch can be created...

...photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room for improvement...

...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...

...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

...best performer mGold — while maintaining high brightness and fast maturation time (Figure 1). See the ...

...G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems for genome engineering...

...McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera...

...BioMolecular Modeling, we spun off a small, family-run business – 3D Molecular Designs – to commercialize some...

...these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...

...Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software produces quantifiable...

...science outreach: engaging the public to promote an awareness and understanding of science. Like most science...

...the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a...

...interview or campus visit in person, things like sickness or scheduling issues can and do interfere with...

...you like to bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me...

...website to verify plasmids or validating your new clones, try these tips to make the most of your time and...

... new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction...

...Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation...

... of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for each paper...

...parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in...

...transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ...

...place to work, we do SO many fun things!!”  Okay - honestly, maybe not everyone replies with quite so much...

...Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting...

... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...

...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...

...Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources...

...You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ...

... your gene to be in relation to the rest of the genes? And do you want it to be in the same (forward) ...

...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...

...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

... Common negative controls include knockout cell lines or tissues that do not express the protein. With...

...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

...pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266...

...Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ...

...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...

...make stricter gates while some like to make looser ones (Figure 3). A more “liberal” gate in the beginning...

... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...

...Western blotting When comparing samples in different lanes in a western blot, you’ll need to include a loading...