We narrowed to 502 results for: Ank

1. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ...collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most...

2. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ...your work, head over to greenfluorescentblog. Thank You to Our Guest Blogger! Gal Haimovich, PhD, is...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...dynamics of PPIs are the focus of study. Many thanks to our guest blogger Patrick Miller-Rhodes from...

4. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   ...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   ... results for China with more uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin...

6. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   ...to choose  Where is your audience?  It’s, quite frankly, much easier to go where your audience is than ...

7. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   ...in bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor...

8. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...Check out the NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant...

9. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   ...from a client you helped. Maybe you awkwardly say thanks and move on with your day, feeling good but focused...

10. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...homologous sequences to the insert by PCR. Many thanks to our guest bloggers Jake Watson and Javier García-Nafría...

11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ... In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced...

12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ...-L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh Karve! Aneesh Karve...

13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions...

14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    ...rodents. In this test, an animal is placed into a tank of water with no support from below or anything ...

15. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ...likely to continue with help from zebrafish. Many thanks to our guest blogger Katherine Rogers from the ...

16. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...and experiences in the comments below.   Many thanks to our guest blogger Samuel Mortensen from Northeastern...

17. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...experimental goals. If not, give CRISPRa a go! Many thanks to our guest blogger Patrick Miller-Rhodes from...

18. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ... protein overexpression might skew the results. Thanks, stringent regulation! This post was originally...

19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    ...patient-derived iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References...

20. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    ...Ikemura lab created the Codon Usage Tabulated from GenBank database (CUTG), that provides an electronic dataset...