We narrowed to 503 results for: Ank;

1. Tips for Using BLAST to Verify Plasmids

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   ...collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most...

2. Which Fluorescent Protein Should I Use?

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   ...your work, head over to greenfluorescentblog. Thank You to Our Guest Blogger! Gal Haimovich, PhD, is...

3. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...in bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor...

4. Summer SciComm Series: Modes of Communication

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   ...to choose  Where is your audience?  It’s, quite frankly, much easier to go where your audience is than ...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...dynamics of PPIs are the focus of study. Many thanks to our guest blogger Patrick Miller-Rhodes from...

6. CRISPR 101: RNA Editing with Cas13

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   ...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

7. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ... results for China with more uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin...

8. Plasmids 101: Secondary Nanobody Toolbox

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   ...Check out the NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant...

9. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...from a client you helped. Maybe you awkwardly say thanks and move on with your day, feeling good but focused...

10. Simplify Cloning with in vivo Assembly

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    ...homologous sequences to the insert by PCR. Many thanks to our guest bloggers Jake Watson and Javier García-Nafría...

11. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ... In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced...

12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...-L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh Karve! Aneesh Karve...

13. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions...

14. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...functions, and how we can help keep it healthy. Thank you to Dr. Yoav Ben-Simon for reviewing this post...

15. Plasmids 101: Stringent Regulation of Replication

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    ... protein overexpression might skew the results. Thanks, stringent regulation! This post was originally...

16. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...systems a try, we’d love to hear your feedback!   Thank you to our guest bloggers! Qi Li obtained her Ph.D...

17. How to Write a Scientific Review Article

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    ...conserved domains. This information was available in GenBank, but it was useful to present it all in one place...

18. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ....1231143  Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

19. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...Synthego blog for more great CRISPR content. Many thanks to our guest blogger, Jessica Roginsky. Jessica...

20. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ... pCXWB-EBNA1 37624 transient EBNA-1   Many thanks to our guest blogger, Kusumika (Kushi) Mukherjee...