We narrowed to 499 results for: Ank;

1. Which Fluorescent Protein Should I Use?

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   Blog Post

   ...your work, head over to greenfluorescentblog. Thank You to Our Guest Blogger! Gal Haimovich, PhD, is...

2. Adeno-associated virus (AAV) Guide

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   Guide

   ...consists of two open reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs)....

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...dynamics of PPIs are the focus of study. Many thanks to our guest blogger Patrick Miller-Rhodes from...

4. CRISPR 101: RNA Editing with Cas13

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   ...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   Blog Post

   ... results for China with more uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin...

6. Summer SciComm Series: Modes of Communication

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   Blog Post

   ...to choose  Where is your audience?  It’s, quite frankly, much easier to go where your audience is than ...

7. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...Check out the NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant...

8. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...in bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor...

9. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ... In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced...

10. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...from a client you helped. Maybe you awkwardly say thanks and move on with your day, feeling good but focused...

11. Simplify Cloning with in vivo Assembly

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    ...homologous sequences to the insert by PCR. Many thanks to our guest bloggers Jake Watson and Javier García-Nafría...

12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ...-L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh Karve! Aneesh Karve...

13. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions...

14. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...patient-derived iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References...

15. To Codon Optimize or Not: That is the Question

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    ...Ikemura lab created the Codon Usage Tabulated from GenBank database (CUTG), that provides an electronic dataset...

16. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ....1231143  Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

17. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ...systems a try, we’d love to hear your feedback!   Thank you to our guest bloggers! Qi Li obtained her Ph.D...

18. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ...rodents. In this test, an animal is placed into a tank of water with no support from below or anything ...

19. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post

    ...likely to continue with help from zebrafish. Many thanks to our guest blogger Katherine Rogers from the ...

20. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...and experiences in the comments below.   Many thanks to our guest blogger Samuel Mortensen from Northeastern...