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We narrowed to 771 results for: Dos

Showing: 421 - 440 of 771 results

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...Choosing a topic and gauging interest The first thing to do if you’ve thought about starting a journal club is...make. How often will you hold it? How many people do you expect to attend? What is the expectation on ...

  4. Pooled Library Amplifications

    Type
    Blog Post
    ... = 500,000 colonies.  Pro tip! Since you probably do not want to individually count 500,000 colonies, ...did not result in significant recombination. If you do see recombination, we recommend ensuring the cells...

  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries)? ...scientists use CRISPR technology? We think so — and so do our requestors! CRISPR plasmids in the Addgene repository...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...they are present at a very low concentration. So how do we make sure to catch all of these potential mishaps...expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression ...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   ..., you will not be able to use far red-FPs. If you do not have a filter that will pass blue light to the...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ... homology arms and EGFP are available at Addgene. Do you have suggestions for other plasmids that should...

  10. Lentivirus Plasmids

    Type
    Collection
    ...Lentiviral Preps Content last reviewed: 23 October 2025 Do you have suggestions for other plasmids that should...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...offer tools to help with these decisions.  So when do you want to codon optimize your gene of interest?...useful in expressing functional proteins in hosts that do not naturally express that gene. The protein you ...

  12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...negative control to determine whether your PIPs actually do interact. Choosing the right negative control is ...scenarios that call for a simple, yes-no answer — i.e., do two proteins interact or not? — FRET is preferable...endogenous PIPs should decrease the BiFC signal in a dose-dependent manner. This is straightforward in easy-to-transfect...easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also theoretically...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...time, when scientists who support open science, but do not share their own data - which seems to be the ...members or colleagues: How much unpublished AAV data do you have sitting in computers and notebooks right... gene transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy...

  15. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... beads are particularly advantageous because they do not require centrifugation between washes which can...controls include knockout cell lines or tissues that do not express the protein. With this control set, you...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescence microscopy (Dani, et al., 2010). How do you overcome this resolution limit? Electron Microscopy...Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural relationships...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...life. That’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would...to express your protein of interest. However, you do still need a species-compatible promoter for expressing...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...five of these Cas proteins, while type I-C Cascades do not contain Cas6. Regardless of the subtype, the ...to the multi-Cas mechanism. These benefits of Cas3 do come at a cost — the previously mentioned issues ...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...gradients, practice making the iodixanol layers before doing your first purification. This helped me get a feel...to learn more about different ways to titer AAV. Do you have any AAV production tips or tricks? Please...multiplicity of infections (MOIs), to determine the optimal dose of AAV for my cells of interest. Every batch of ...
Showing: 421 - 440 of 771 results