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We narrowed to 769 results for: Dos

Showing: 421 - 440 of 769 results

  2. Pooled Library Amplifications

    Type
    Blog Post
    ... = 500,000 colonies.  Pro tip! Since you probably do not want to individually count 500,000 colonies, ...did not result in significant recombination. If you do see recombination, we recommend ensuring the cells...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries)? ...scientists use CRISPR technology? We think so — and so do our requestors! CRISPR plasmids in the Addgene repository...

  4. Of Myc and Men

    Type
    Blog Post
    ...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take... to be less sensitive to sequence context.    So, do Schüchner, et al.’s results mean the end of 9E10’...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...Choosing a topic and gauging interest The first thing to do if you’ve thought about starting a journal club is...make. How often will you hold it? How many people do you expect to attend? What is the expectation on ...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   ..., you will not be able to use far red-FPs. If you do not have a filter that will pass blue light to the...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ... homology arms and EGFP are available at Addgene. Do you have suggestions for other plasmids that should...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluoresce, the Jaffrey lab asked:  Could an RNA aptamer do the same thing? Using the HBI derivative 3,5-difluoro...recognition of ions or particularly small molecules that do not generate an immune response. Aptamers can be ...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...offer tools to help with these decisions.  So when do you want to codon optimize your gene of interest?...useful in expressing functional proteins in hosts that do not naturally express that gene. The protein you ...

  12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...negative control to determine whether your PIPs actually do interact. Choosing the right negative control is ...scenarios that call for a simple, yes-no answer — i.e., do two proteins interact or not? — FRET is preferable...endogenous PIPs should decrease the BiFC signal in a dose-dependent manner. This is straightforward in easy-to-transfect...easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also theoretically...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...time, when scientists who support open science, but do not share their own data - which seems to be the ...members or colleagues: How much unpublished AAV data do you have sitting in computers and notebooks right... gene transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy...

  15. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... beads are particularly advantageous because they do not require centrifugation between washes which can...controls include knockout cell lines or tissues that do not express the protein. With this control set, you...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular biologists working with ...in order to understand what these vector particles do once they come in contact with target cells (Fig....

  17. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescence microscopy (Dani, et al., 2010). How do you overcome this resolution limit? Electron Microscopy...Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural relationships...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...life. That’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would...to express your protein of interest. However, you do still need a species-compatible promoter for expressing...

  19. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...five of these Cas proteins, while type I-C Cascades do not contain Cas6. Regardless of the subtype, the ...to the multi-Cas mechanism. These benefits of Cas3 do come at a cost — the previously mentioned issues ...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...
Showing: 421 - 440 of 769 results