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We narrowed to 772 results for: Dos

Showing: 421 - 440 of 772 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    ... = 500,000 colonies.  Pro tip! Since you probably do not want to individually count 500,000 colonies, ...did not result in significant recombination. If you do see recombination, we recommend ensuring the cells...

  2. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries)? ...scientists use CRISPR technology? We think so — and so do our requestors! CRISPR plasmids in the Addgene repository...

  3. Of Myc and Men

    Type
    Blog Post
    ...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take... to be less sensitive to sequence context.    So, do Schüchner, et al.’s results mean the end of 9E10’...

  4. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...they are present at a very low concentration. So how do we make sure to catch all of these potential mishaps...expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression ...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...use. This will prevent spills, but, if any spills do occur, they should be wiped with 70% ethanol immediately...morphologies, being familiar with these morphologies and doing a quick check under the light microscope will help...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...Choosing a topic and gauging interest The first thing to do if you’ve thought about starting a journal club is...make. How often will you hold it? How many people do you expect to attend? What is the expectation on ...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   ..., you will not be able to use far red-FPs. If you do not have a filter that will pass blue light to the...

  14. Ras Pathway

    Type
    Collection
    ...Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions for other plasmids that should...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should...

  16. Lentivirus Plasmids

    Type
    Collection
    ...more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids that should...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...life. That’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would...to express your protein of interest. However, you do still need a species-compatible promoter for expressing...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...five of these Cas proteins, while type I-C Cascades do not contain Cas6. Regardless of the subtype, the ...to the multi-Cas mechanism. These benefits of Cas3 do come at a cost — the previously mentioned issues ...

  19. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...

  20. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular biologists working with ...in order to understand what these vector particles do once they come in contact with target cells (Fig....
Showing: 421 - 440 of 772 results