We narrowed to 472 results for: FLU;

1. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   ...The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to isolate differentiated...

2. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   ...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

3. Antibodies 101: Immunoprecipitation

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   Blog Post

   ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  Resources...

4. QC Sequencing Technologies at Addgene

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   Blog Post

   ...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

5. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   Blog Post

   ...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

6. Plasmids 101: Shuttle Vectors

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   Blog Post

   ...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

7. Viral Production at Addgene

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   Blog Post

   ...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

8. Twenty Years of Sharing: Addgene's Viral Vector Service

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   Blog Post

   ...launch of the redesigned Data Hub in 2022. It’s influenced the way we approach our viral vector educational...

9. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   ... number of iterons encoded on a given plasmid influences how high the copy number needs to get before ...

10. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ...vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ...

11. Using Video to Share Your Science: We Share Science

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    Blog Post

    ...own work. Of course not all research has to be influenced by research in other disciplines. But there remains...

12. CRISPR Activation: A Practical Guide

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    Blog Post

    ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ... respondents indicated that ease of use would influence their ELN choice, with almost 80% rating it as...

14. Five Popular Model Organisms, Part 2

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    Blog Post

    ...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including...

15. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ... unnatural head groups for applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD...

16. Tips for Getting a Faculty Position

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    Blog Post

    ...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

17. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...generally preferred for large insertions such as fluorescent proteins or selection cassettes. This class of...

18. Plasmids 101: Biotinylation

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    Blog Post

    ...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

19. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

20. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    ...right tags to your gene, and may have even put a fluorescent protein downstream, separated by an IRES element...