We narrowed to 476 results for: FLU;
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TypeBlog Post...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...
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Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...launch of the redesigned Data Hub in 2022. It’s influenced the way we approach our viral vector educational... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post... number of iterons encoded on a given plasmid influences how high the copy number needs to get before ... -
Viral Production at Addgene
TypeBlog Post...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre.... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts Resources... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...mouse adenovirus in mouse; HIV1, West Nile, and Influenza in humans), LY6A lacks a defined role in viral... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to isolate differentiated... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...in the Endoplasmic Reticulum, optimization of fluorescent proteins, and live cell imaging approaches. ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...several previously observed characteristics that influence gRNA off-target activity: 1) mismatches closer... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...scientists have studied how different parts of MECP2 influence Rett Syndrome. Zoghbi’s lab found that a small... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...own work. Of course not all research has to be influenced by research in other disciplines. But there remains... -
CRISPR Activation: A Practical Guide
TypeBlog Post... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post... unnatural head groups for applications like fluorescent labeling via click chemistry. Figure 4: SuperPLD... -
Tips for Getting a Faculty Position
TypeBlog Post...in the Endoplasmic Reticulum, optimization of fluorescent proteins, and live cell imaging approaches. ... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...generally preferred for large insertions such as fluorescent proteins or selection cassettes. This class of... -
Plasmids 101: Biotinylation
TypeBlog Post...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post... respondents indicated that ease of use would influence their ELN choice, with almost 80% rating it as...