We narrowed to 877 results for: HA

...preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for ...mosaic virus, the constitutively active M24 promoter has been modified with duplicated enhancer domains and...

...keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our...the same time, he pointed out, academic research has some big problems resulting from past practices and...

...form only weak and reversible bonds. Howarth’s team has already demonstrated in their PNAS paper that SpyLigase...discovered the domain. It is small, highly stable and has this amazing chemistry locking the domain together...

...research project can take several years to complete and has multiple benchmarks. Think about what your personal...Have a look at what others have done recently that has been a big hit, and especially look at the new tools...

...overexpression. Tim Formosa, at the University of Utah, has built a complete collection of yeast tagging modules...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site...

...Toronto KnockOut (TKO) library from the Moffat lab has over 175,000 different gRNA-containing plasmids)....

...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

... its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in popularity with an abundance...target concentration during expansion. One group has found that using Fab fragment secondaries, rather...experimental approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of...

... codon table — a chart every molecular biologist has seen before, showing how DNA sequences are translated...lost from the population. A bacterial chromosome has a single origin of replication (ori), where DNA replication... you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even in cells with ...

...studying mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult...editing (PE), initially developed in human cells, has been uniquely challenging to apply in dicotyledonous...

...depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus has a capacity of 8–10 kb...., or reagents and equipment. Hopefully this post has provided some insight on these differences in viral...

...et al., 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions ...yet to be demonstrated (or invented), its behavior has already been successfully reproduced by several other...tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

...agents. Directly conjugating the primary antibody has the additional benefit of permanent linkage. This...steps following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak... the scope of this post.  We hope that this blog has provided you with a useful overview of the IP method...

...your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-...edited.   As discussed above, the ability to edit RNA has multiple advantages, including reversibility and ...

...months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated...contains requests from 5/22/14 to 5/19/20). Each paper has between 1–32 plasmids and a total of 2–2,590 requests...

...Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...often under-appreciated or over-looked.  Synthego has developed a new tool called ICE (Inference of CRISPR...increased flexibility and scalability, the ICE software has two analysis formats: sample by sample analysis, ...

... a primary antibody. B) Each arm of the antibody has an antigen binding site for a total of 2 binding ...  Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your...refers to the number of cysteine sites the nanobody has available for conjugation to a specific label (see...

... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...using the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that ...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...

... following IV injection. Compared to AAV9, PHP.B has a >40-fold increase in CNS transduction and transduces...adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 gene... line of viral vector permissiveness While PHP.B has impressive CNS transduction in some strains of mice...

...fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection...putative T1 transformants: When your T1 is on soil and has recovered from the stress of selection you can clip...cell fate maintenance in the stomatal lineage and has made countless transgenic plants in the process. ...