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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 421 - 440 of 856 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...et al., 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions ...yet to be demonstrated (or invented), its behavior has already been successfully reproduced by several other...tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

  2. Tips for arabidopsis transformation

    Type
    Blog Post
    ...fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection...putative T1 transformants: When your T1 is on soil and has recovered from the stress of selection you can clip...cell fate maintenance in the stomatal lineage and has made countless transgenic plants in the process. ...

  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...properties of modified histones, a genomic region that has mono-methylated and acetylated histones, but not ...magic and Poisson statistics, each sub-population has, on average, one distinct enhancer disrupted. As ...unhealthy cells. Until recently, the human genome has been full of dark matter: enhancers, lncRNAs, repetitive...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...the State Museum has been the best supporter and most productive partnership CINSO has had. Volunteer! ...provide online. Twitter is an excellent resource that has gained a lot of traction with researchers. It is ...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...cells where binding between the proteins of interest has occurred. Although the original Y2H systems utilized... number of different bait and prey vectors. This has been shown to be as effective as using five independent...transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban M.J.,...

  6. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...but the introduction of type V Cpf1-driven systems has added another option to the CRISPR toolbox. Cpf1’...frequency of desired HDR edits, but the possibility has not yet been experimentally confirmed. To prevent...adaptation of Cas13 to RNA targeting and RNA editing, has further shown the diversity of CRISPR-based systems...

  7. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...plasmid we have at Addgene. Each sample at Addgene has four layers of location which can all be extrapolated...hold up to 96 tubes (see image below). Each plate has numbers and letters to give coordinates, from A1 ...replicate sample. The transformation After the DNA has been received and stored, it is put into E. coli ...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...cleavage with some of these peptides, no consensus has been reached on which peptide works best. Likely ... is easiest to start with a plasmid that already has the multicistronic element and reporter cloned in... unique genes, you can start with a plasmid that has multiple cloning sites flanking the multicistronic...

  9. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... Lab technician / Research assistant Typically has a Master's or Bachelor's degree Generally a short-term...talking to people, though, try to be conscious of who has your best interests at heart. A great piece of advice...vulnerable about my struggles and what I don’t know yet has helped me cultivate stronger relationships with my...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career options...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...extraordinary collection of talent, of human knowledge, that has ever been gathered at the White House - with the ... not an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  ... on a leaderless team. Sometimes the leader just has to be the person to say “OK, guys, we need to have...anything out the discussion themselves, then one has to question whether that person should be on the ...

  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... technique developed in David Liu’s lab in 2019, has added considerably more precision and flexibility... the canonical base editing window, base editing has higher efficiency and fewer indels than prime editing... repair (HDR) stimulated by double strand breaks has been widely used to generate precise changes. However...nicking of the unedited strand only after editing has occurred and reduces indels by 13-fold (Anzalone ... al., 2024; Yarnall et al., 2023) Prime editing has a lot of promise for treating genetic diseases. In...

  12. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...techniques and troubleshooting tips. The fourth edition has been fully updated with twelve new sections, including...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...on single-cell gene expression patterns. The lab has also used CellTags for tagging two cell populations...libraries at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...

  19. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

  20. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... power of science to improve the world around us has long been known; ensuring all have access to this...information becomes easier to access by the day, it has never been more crucial to understand just how we...
Showing: 421 - 440 of 856 results