...buffer such as TE. You will now have plasmid DNA that has been purified away from the bacterial proteins and...

... technique developed in David Liu’s lab in 2019, has added considerably more precision and flexibility... the canonical base editing window, base editing has higher efficiency and fewer indels than prime editing... repair (HDR) stimulated by double strand breaks has been widely used to generate precise changes. However...nicking of the unedited strand only after editing has occurred and reduces indels by 13-fold (Anzalone ... al., 2024; Yarnall et al., 2023) Prime editing has a lot of promise for treating genetic diseases. In...

...techniques and troubleshooting tips. The fourth edition has been fully updated with twelve new sections, including...

.... Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated one... available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while... SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence... create the adenine base editor. The TadA domain has also been further evolved to catalyze cytidine deamination...editing requires more optimization and typically has lower efficiency (< 50%) than base editing (> 90%...modifications to eliminate target gene function. It has also been used extensively to screen for novel genes... those for yeast or drosophila . Although CRISPR has been less widely used in bacteria due to technical...

...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics...

...Throughout my internship, my interest in scicomm has grown and now it feels like an awesome bionerd hobby...Networking Tips Here And when asked what tips he has for better science communication for non-scientists...

...in Biochemistry with a background in virology who has worked with both animal and plant viruses. Now focusing...compounds with various antimicrobial activities (1). She has developed a fast endophyte screening system and plans...

...overlap extension PCR. Although each type of cloning has its advantages, most scientists still encounter several...science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning...

...on single-cell gene expression patterns. The lab has also used CellTags for tagging two cell populations...libraries at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...

...single-molecule super resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent...fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks (max excitation...

...product packaging) for free! It has to be from their company and it has to be packaging (no coated tissue...

...beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified...predictors because they are just that: rare. “Nature has many genes where there are not rare codons and many...

...this crisis has highlighted the importance of access to quality materials and data. Addgene has some exciting...

...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

... power of science to improve the world around us has long been known; ensuring all have access to this...information becomes easier to access by the day, it has never been more crucial to understand just how we...

...the field and terminology that is widely used but has a very specific use-case (definition) in a specific...specific field.  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the ...

...large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright red pigment... University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful imaging.   ...

...available, the next best thing is a protein that has been validated for an assay that uses similar sample...Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western because...

...protein that no other protein in the expression system has. This is where affinity tags come in – they are peptides...solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...

...scheme, known as the Baltimore replication classes, has been developed around how they replicate (Figure ..., which retains the protein coat (and envelope), has a “gutted” genome with genes central to the replication...