We narrowed to 771 results for: LIS
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TypeBlog Post...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...not map to the reference sequence. The resulting list of hits is then carefully reviewed. As mentioned...
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PCR Applications for Copy Number Variation Assays
TypeBlog Post... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post... PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for...PCR is that it can amplify DNA using only a short list of reagents and several heating and cooling steps... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...not better, than antibodies for all of the above listed molecular techniques, but they can also be expressed... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ... -
MXS Chaining
TypeBlog Post...The tradeoffs between various cloning methods are listed in Table 2. For example, chaining-based cloning...used in various chaining-based cloning methods are listed in Table 3. The goal of high throughput cloning... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post... to reflect upon before they attempt to make essentialist connections between biology and ancestral groups...the long and ugly history of reporting false essentialist links between biology and ancestry group as ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version... See Supplementary Table 3 in Huang et al. for a list of PHP.B permissive and non-permissive mouse strains... -
Lab to Office Culture Shock
TypeBlog Post...polling some Addgene colleagues, we’ve come up with a list of things to consider as you make this transition...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK... -
Tips for Writing a Good Cover Letter
TypeBlog Post...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am... -
Transferable Skills Guide: Creativity
TypeBlog Post...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post... what institutions are actually doing (using the list of 340 institutions with postdocs in science, engineering... attempted to check 123 of the 340 institutions listed as having postdocs, representing ~85% of the postdoctoral... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...Experiment with New Careers while in Grad School Listen to Michael Koeris' Journey from Grad Student to... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly...