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We narrowed to 840 results for: Met

Showing: 421 - 440 of 840 results
  1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post
    ...viral production strategies or developing novel methods for data analysis, our team’s goal is to provide...and laboratories alike as an easy and inexpensive method to improve AAV yields. Although we only investigated...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...
  2. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post
    ...light. In a recent article published in Nature Methods, Boyden and his team describe the discovery of ... tree of life. Our most recent paper in Nature Methods offers the first true two-color pair. We'll be ...Neural Populations. Nathan Klapoetke et al. Nature Methods. 2014 Feb 9. (PubMed). Read More: Other Optogenetics...
  3. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ...rate that study as untrustworthy, inaccurate, methodologically flawed, and biased. As a result, the control...because a greater familiarity with scientific methodology makes it easier to spot even minor weakness or...might struggle to understand. Why should we trust something that we cannot read or verify for ourselves? Further...
  4. 25 Work From Home Tips for Scientists

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    Blog Post
    ...support your favorite local exercise spot or find something new. We usually have yoga class twice a week at...more independently. For example, they could do something like puzzling or crafts, while listening to podcasts...registered report, where peer review is based on the methods, not the results. Also consider using this framework...
  5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ... more effective mAb therapeutics. The in silico method of immunogenicity assessment can help in determining...All approaches have their own limitations. Using methods together can inform decisions about in vivo application...for Sequences Containing Unnatural Amino Acids: A Method Using Existing in silico Algorithm Infrastructure...
  6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples on dry...following transport or storage on dry ice. Nat Methods. 2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2...recombinant adeno-associated virus vectors. Mol Ther Methods Clin Dev. 2014 Aug 13;1:14034. PubMed PMID: 26015974...
  7. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    ... biosensors, which are optimized for specific parameters: jGCaMP7s is the most sensitive responder to...primary inhibitory neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal...Optogenetics Optogenetics continues to be a useful method for manipulating specific neurons. We recently ...
  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    Blog Post
    ...exported as a picture. I believe that showing something different from time to time breaks up the monologue...monologue a little and gives participants something else to focus on. Use collaborative cloud apps It is... Take brain breaks Meetings can get very long sometimes, and interaction fades away the longer we are ...
  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post
    ... et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision. Our...or double stranded donor DNA with other design parameters. Example single stranded donor DNA and a presumptive...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...
  10. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ...probe and build new systems. Such goals require methods to directly activate and inactivate biological ...that prints a chemical image, as well as a genetic method for the computational problem of edge detection...identified in bacteria, and with similar optimization methods, many of these systems could be made suitable for...
  11. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ...variety of cloning techniques and troubleshooting methodologies drew me to learn more about the process that...immunofluorescence-immunocytochemistry (IF-ICC.) This method was chosen because cells transfected with a plasmid...available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for construction and...
  12. Hot Plasmids - February 2022

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    Blog Post
    ...Clouse While many CRISPR-based genetic screening methods have primarily used Cas9 to knock out genes, researchers... screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability to perform multiplex...in parallel. Lastly, this Cas12a-based screening method was used to create a genome-wide library capable...
  13. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ... a look at a few screening methods. Blue-white screen A widely used method of screening is the blue-white...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...
  14. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...gene a megabase away, or looking at the histone methylation status at a double-strand break that needs repairing...guest bloggers have written posts describing new methods and tools for studying chromatin and the epigenome... and Editing the Epigenome CUT&RUN: An Improved Method for Studying Protein-DNA Interactions CRISPRainbow...
  15. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post
    ...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ... al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated...
  16. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a .... And, naturally, rarely are the optimization parameters the same for all three components, meaning that...Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural Amino...
  17. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...understand how the brain works. There are two methods of neuronal tracing (Fig. 1): one to visualize ...catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies...neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon is...
  18. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post
    ...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... depending on your interests. You can look at something as basic as survival. For those interested in ...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...
Showing: 421 - 440 of 840 results