...you are spending on a conference? One thing to consider is the size of the conference. Large conferences...favor small conferences where the attendees stay onsite and eat together because scientists have time to...– even students and postdocs. There are many inexpensive/free places to order cards. There are a few good...

...publication in a scientific journal requires several considerations. First, it is important to establish the type...journal. An increasing number of funding bodies insist that the research data resulting from their funding...is a Developmental Editor for F1000Research, responsible for assessing the scientific content of manuscripts...

...sequencing and qPCR methods are too costly and time intensive to process a large number of microbiome samples...bumble bee and honey bee microbiome. The toolkit consists of modular parts that are assembled with Golden...microbial population dynamics in the gut can provide insight into how the microbiome responds to infection, ...

...red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent... Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N...existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage...

...antibody, this approach can greatly increase the sensitivity of an assay by increasing the number of signaling...place over multiple days. Increased run time is considered one of the major drawbacks of the indirect method...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...

...advantage of polyclonal antibodies is that they are inexpensive. Their short production time and the bulk purification...labs. Polyclonal antibodies also have a high sensitivity to the target protein due to their ability to...or fixation (IHC). Because of their increased sensitivity, polyclonal antibodies are particularly useful...

...might use it in the lab. What is a Fab Fragment? Consider the classic IgG antibody (Figure 1): it is composed...new components in your antibody tool box. As you consider which tools to use, be sure to think about what... other components of your experiment. These considerations will help you narrow down which antibody format...

...organized lab saves time and money across the board. Consider alternative cloning strategies There are many ...methods, like Gibson assembly, are typically more expensive, so you’ll have to find a balance between time...either don’t scale or have limited scaling. The downside of high-throughput work is that you give up a ...

... dsDNA. It cleaves ssDNA with high fidelity - sensitive to even a single mismatch in the target sequence...This makes it particularly useful in generating transient edits of signaling molecules (RNA) as opposed ...for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This system could...

...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...during your postdoc that you enjoy and have a propensity for cross team communication, that’s an important...

...negative regulation by using a promoter that is responsive to a repressor protein, the increase in expression... of repressor protein also increases thereby intensifying repression of the gene of interest and keeping...the increase in plasmid copy number during the transition to stationary phase, Segall-Shapiro et al. placed...

...that consistently receives around 200 new DNA samples every week. Here we will provide an inside look ...terminators. Detailed instructions on other factors to consider when designing your primers can be found here....

...site-specific recombination events. The system consists of two components derived from the P1 bacteriophage...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...

...science and collaboration. The field already has comprehensive reference pages and curated databases for scientists... (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every C.elegans  neuron...Scientists monitor the effects of RNAi using four-dimensional contrast interference (DIC) microscopy and computer...

...faster assembly and disassembly dynamics, higher sensitivity to light, and better distribution of clusters...measure fluctuations of TagRFP-T fluorescence intensity due to interactions with photoactivatable fluorescent...By monitoring changes in TagRFP-T fluorescence intensity at different time points, the authors tracked ...

...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

...position, I just had a phone interview. I also transitioned between different lab groups without having ...that have lots of general questions about job responsibilities, performance evaluations, professional development...difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a backup, if the company...

... this type of ELISA is extremely specific and sensitive to the target protein due to the use of multiple...concentrations of your protein of interest. The optical density (OD) - the measure of light absorbed by a solution...any experimental wells in that range of optical densities. If your standard curve does not have a linear...

...article we will discuss general viral safety considerations and best practices for handling lab-grown viruses...replicate and continue the infection cycle once inside the host. While the virus can still enter (and ...into the host’s DNA. These viruses also have a propensity to integrate into proto-oncogenes, which can ...

...significant plasmid elements. Which differences are considered a QC issue is reviewed yearly at a best practices...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...assembly in Geneious can still provide additional insights to resolve the assembly and determine if there...