We narrowed to 588 results for: RET

1. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...transgene is flanked by two ITRs, and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector...

2. "Build Your Plasmid": The Game - Play to Teach

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   Blog Post

   ...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...

3. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ... nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate untranslated mRNAs...

4. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...highly optimized bioinformatics pipeline that will return high-quality assembled and circularized plasmids...

5. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer lentivirus...

6. Hot Plasmids - January 2023

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   Blog Post

   ...consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl...

7. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   Blog Post

   ... mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying a reagent...

8. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   ...force-generating protein machines that can extend and retract over many microns in response to pH changes without...

9. Components of CRISPR/Cas9

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   Blog Post

   ...repeated elements “direct repeats” and the unique stretches of DNA between them “spacers” (see figure below...

10. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...proteins, imaging four colors simultaneously should be pretty straightforward. Thank You to our Guest Blogger...

11. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post

    ...dye! Always run a ladder. Ladders allow you to interpret the bands that you get in your sample lanes. Choose...

12. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

13. Hot Plasmids - May 2022

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    Blog Post

    ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

14. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

15. Antibodies 101: Isotypes

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    Blog Post

    ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

16. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

17. Targeted Mutagenesis with EvolvR

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    Blog Post

    ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...

19. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...

20. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...