We narrowed to 583 results for: RET
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TypeBlog Post...recommendation for the correct statistical test can save a retraction. Today, collaborations are limited by the difficulty...
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Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...transgene is flanked by two ITRs, and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...time to network,” Zhang says. “The schedule was pretty busy, but after the meeting, I received a lot of... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou... -
25 Work From Home Tips for Scientists
TypeBlog Post...defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the... -
Hot Plasmids - January 2023
TypeBlog Post...consider hfYFP and LSSmGFP. Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...aliquots in AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific serotypes ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...force-generating protein machines that can extend and retract over many microns in response to pH changes without... -
Components of CRISPR/Cas9
TypeBlog Post...repeated elements “direct repeats” and the unique stretches of DNA between them “spacers” (see figure below... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post..., does not require standard curves, and can theoretically detect a single target molecule in a sample.... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ... -
Targeted Mutagenesis with EvolvR
TypeBlog Post... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries... -
Hot Plasmids - May 2022
TypeBlog Post...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab... -
Antibodies 101: Isotypes
TypeBlog Post... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...