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We narrowed to 754 results for: REV

Showing: 421 - 440 of 754 results
  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...
  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...
  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture...proteins at endogenous loci, shedding light on previously unknown protein functions. References Schwartz...
  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...
  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...large field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features...
  6. An Introduction to Adenovirus

    Type
    Blog Post
    ... vector can still occur even in the case of no previous immunity existing for that adenoviral vector (...(2019). Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679...
  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...more accessible way to image biological features previously only visible via techniques like super-resolution... disciplines now available. In this post, we’ll review the inspiration behind ExM, how it works, and some...
  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...
  9. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...large number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many... to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman in ...
  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity and accuracy...recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation...
  11. AAV Production in HEK293 Cells

    Type
    Protocol
    ... time. Allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...medium and pool with the cells harvested in the previous step. Pipette back and forth or shake vigorously...split 2 days before these images were taken. Last reviewed on: October 10, 2023...
  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate...gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9...
Showing: 421 - 440 of 754 results