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We narrowed to 741 results for: REV

Showing: 421 - 440 of 741 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    ... vector can still occur even in the case of no previous immunity existing for that adenoviral vector (...(2019). Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...

  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...more accessible way to image biological features previously only visible via techniques like super-resolution... disciplines now available. In this post, we’ll review the inspiration behind ExM, how it works, and some...

  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...large number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many... to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman in ...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ... time. Allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...medium and pool with the cells harvested in the previous step. Pipette back and forth or shake vigorously...split 2 days before these images were taken. Last reviewed on: October 10, 2023...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ... blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...considerably easier, faster, and more efficient than previous methods. Still, generating reliable results from...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate...gRNAs into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...require a PAM, and RNA targeting is potentially reversible since there is no genomic edit. Some Cas13 enzymes...KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...involves insertion of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition...

  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...conversion by engineered glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143...

  17. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... you will not be a graduate student or postdoc forever, and you need to be thinking about what comes next... development center, if one exists. Get online! Review company and institution career pages. Make yourself...

  18. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...takes on cases requiring in-depth analysis, data review, in-house testing etc. Technical support roles ...too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting protocols,...

  19. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): 434–464. Pubmed PMID: 9618448 Diaz R, Rech...RP (1987) Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793 Schumacher...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...the questions.  After the code is written, it is reviewed by at least one other developer. This is another...point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into the mind of...
Showing: 421 - 440 of 741 results