We narrowed to 537 results for: SHI

1. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   ...-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, turning expression...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ... inserted or deleted, can result in an in-frame shift of mCherry and thus expression. As a proof of concept...

3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   ... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...

4. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ...’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures, tons of ...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   ...blue light which revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead...

6. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   ...the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted...

7. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   ... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

8. Antibodies 101: ChIP

   Type

   Blog Post

   ...alongside your input control. Elute the DNA after washing and then incubate with Proteinase K to reverse ...

9. Hot Plasmids - February 2022

   Type

   Blog Post

   ...frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently published a set of ...

10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ...protein (EBFP2) is expressed in an unregulated fashion and thus a marker for transfection. The other (...

11. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    ...thanks to our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has...

12. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    ... plasmids from this paper at Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent...

13. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ...consequently about a two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can...

14. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    ...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    ...desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the...

16. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...

17. The time and cost required to make a plasmid

    Type

    Blog Post

    ...things go wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up...

18. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate protein...

19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory...

20. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    ...screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage...