We narrowed to 532 results for: SHI

...protein (EBFP2) is expressed in an unregulated fashion and thus a marker for transfection. The other (...

... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

...-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, turning expression...

...alongside your input control. Elute the DNA after washing and then incubate with Proteinase K to reverse ...

...frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently published a set of ...

... inserted or deleted, can result in an in-frame shift of mCherry and thus expression. As a proof of concept...

... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...

...’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures, tons of ...

...the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted...

...consequently about a two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can...

...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

...desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the...

...photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains on either side...

... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

...does not depend on excitation power in a linear fashion. This implies that reducing the power 2-fold does...

...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

...: 30643213. PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic...

...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...

...things go wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up...

...and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate protein...