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  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers need...between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens ...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... journal club? Where will the journal club take place? Preparing for the first meeting There are many ...important pieces before that first meeting takes place to ensure success. One of the most important is ...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...required for a mini amplification reaction to take place.  When the PCR is complete, a droplet reader extracts...contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’s seminal...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...labeling so that a cell’s projections are easy to trace and not lost in a jumble of fluorescent neurons.... But notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...editors Excises G, T, or C, respectively, to be replaced with another base. References Chen, L., Hong...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...through the stomata into the abaxial site (lower surface) of the leaf. This is a simple and easy technique...Mortensen et al., 2019): Leaves or whole plants are placed in an Agrobacterium solution and a vacuum is applied...

  14. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...struggling to understand how the researchers decided to place their gates on the plots! Flow data is messy, so...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...are 27 incompatibility (Inc) groups in the Enterobacteriaceae family alone, and this is expanding as we...expelled antibiotic resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman...

  16. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... and vary in shape and structure. Their porous surface provides a large area for binding, but antibodies... than agarose beads. Proteins conjugate on the surface of magnetic beads, and are therefore completely...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...commercial antibodies can be tethered on the viral surface, expanding the range of targetable cell types. ...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

  19. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...without disrupting plasmid representation. A good place to start is with a protocol for a library of similar...Moderated by the Feng Zhang lab, this is a useful place to ask experimental questions.  Practical Considerations...
Showing: 421 - 440 of 720 results