We narrowed to 634 results for: des.2

...HDR) and how it’s used in CRISPR genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used...inversions, deletions, and translocations. It also provides researchers with many different ways to control...the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation process...experiment? In this post, Addgenie Chari Cortez guides us through some common controls and why you might...

...PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...package. One important concept in FRET experimental design is that the targeting specificity requirements ...codon (often amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural...so small, one can insert them in virtually any desired location in a target protein, though predicted ...it all together The FRET donors and acceptors described above can be paired together to measure a wide...combinations of labeling strategies. FRET experimental design lends itself to creativity and innovation, valuable...

... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...small-molecule fluorescent labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338...select the optimal dye or fluorescent ligand when designing an experiment. You may find yourself defaulting...protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications of this include..., which is an important consideration for assay design (Breen et al., 2016). When selecting dyes, it's...fluorescent donors and acceptors in their product descriptions, or technical manuals. Specific applications...signal-to-noise ratios. Certain dyes have been specifically designed for use in super-resolution microscopy techniques...

...Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An Institutional Perspective... out to me were: Decrease the Number of Postdocs De-incentivize going into PhD programs as the standard...environments. Slide from Paula Stephan’s Talk (All slides can be found here). Importantly, the panel offered... joining long programs if it is obvious that a desired career outcome is not easily achieved. Of note...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

...our SPARC blog post for more details. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP ...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast...intracellular Ca2+ (bottom panels) equipped with destabilized GFP calcineurin reporter (blue) and stained ...

...(S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

...light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit distinct behaviors...spontaneous swimming. References Arrenberg, Aristides B., Filippo Del Bene, and Herwig Baier. "Optical...

...the world’s population, we account for an estimated 2% of global plastic generation. Less than 9% of plastics...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ...

...remained active in up to 30% plasma. Figure 2: Human plasma longevity of SpCas9 vs GeoCas9. SpCas9...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...

...development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd.2016.238 Additional resources...onset) may be best served by this type of targeted design. Alternatively, a disease model can be generated...

...also be easily understood and consistent in design and layout. 2. Be concise in your writing  When writing...

...but also something about how science really works. 2)   Read science writing in magazines or newspapers...research, SciFund is really motivated more by the desire to help close the widening gap between science ...

... temporal activation of a transgene. Figure 2: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis...this post, you’ve got the knowledge to be able to design a construct for the successful and specific expression...

...available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

... stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...PMID: 30356044. PubMed Central PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher A. Voigt. "Deep...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

...majority of deposited materials to study SARS-CoV-2 would be fully open and available to industry requestors... enhances productive collaborations as well as provides visibility for grant-driven research being done...

... sets Taking the time to identify and research 2 – 3 potential professions will highlight which skills...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...or FP) is one of the most important steps in the design of an imaging experiment.  The choice is non-trivial...fusion protein, etc...).  There are many online guides – including an excellent series of posts by Joachim...information about fluorescent proteins.  It was designed with the goal of encouraging user contributions...while also maintaining a relatively strict database design (schema).  Registered users can submit new fluorescent...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...for, the Table of Fluorescent Protein Properties provides a master list of all proteins in the database,...

...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of... recognition are a few methods by which tumors sidestep immune surveillance: Suppressing Function Antigen...target is never the answer to the multi-level barricades put up by cancer. As mentioned in this article...