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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 830 results for: eed

Showing: 421 - 440 of 830 results

  1. Plan Your Experiment

    Type
    Guide
    ...population need to be identical (clonal) or is a mixed population okay? Does the edit need to be homozygous...interference Needs good primer design Western blot Knockouts Activation/interference Needs a good antibody...other organisms. As with most experiments, you first need to have a biological question in mind. Then you ...will help you narrow down which components you’ll need for a given experiment. In the table below, we highlight...dCas9 to be fused to transcriptional activators that need to be within a given range of the transcription ...HEK293) cells. For more difficult cell types, you may need to look into other options. Browse Addgene's collection...cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, you directly...

  3. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ...should our new blog be any different? If you still need an extra push, there’s evidence that laughter really...’s a way to connect with other people. (We don't need evidence for that one, do we?) OK, so, now that ...

  5. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...though I suspect that our costume contests were indeed occurring earlier but that the photo evidence was...how seriously we take costume design. In case you need more convincing, I’ve got just the proof: Above...

  6. Antibody Guide

    Type
    Guide
    ...process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed with IP..., an antimicrobial agent, can block some amines needed for conjugation reactions and should be removed...chosen. Signal Amplification The amount of signal needed from a specific antibody can vary depending on ...category, including an outline of the basic steps needed for each application, important controls, and any...samples to make proteins available. Tissue samples may need additional processing steps, such as sonication....signal levels. In ChIPseq, an input control will need to be collected before immunoprecipitation. Antibody...from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be...

  7. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...biologists alike. Now with over 600 articles, we needed a better way to organize and present the vast collection...outside of these five areas and you can find what you need by field (ex: microbiology, neuroscience), or explore...

  8. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...choosing the cargo, serotype, and prep size they need for their experiments — and we’ve been able to ship...continue to expand the collection to meet the diverse needs of our community and support a wide range of experimental...

  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...in interactive figures is a great step up, but I needed a way to show different aspects of the data simultaneously... R can further customize the dashboard to their needs. I hope you find the dashboard useful when analyzing...

  10. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...developing a strong graduate student community and the need for belonging in the scientific environment 3:09...graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post Grad Plans 17:07 - 18:29 ...

  12. Adenovirus Guide

    Type
    Guide
    ...therefore needs to be supplied through other means. In order to produce rAdV vectors, E1 needs to be expressed...especially when needing transgenes to express for a shorter period of time or when needing to package larger...starts and primarily encode for structural proteins needed to assemble icosahedral capsids and build new virions...acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at the end... In order to produce rAdV vectors, you generally need two plasmids: Shuttle plasmid (also known as transfer...produced in high titers for their use in experiments. Needle sticks and ripped gloves are common points of entry...system for generating recombinant adenoviruses . Proceedings of the National Academy of Sciences of the United...

  13. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... commitment to be responsive to the community’s needs and our commitment to top notch quality service ...as we grow and change to meet its ever-evolving needs. For now, we have many exciting things planned ...

  14. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ... 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making...’s easy to make your own competent cells. If you need a high efficiency of transformation (for example...

  17. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...writing, and networking Practice your English if you need to, go to ESL classes Practice your presentation... can), form a peer mentoring group to get real feedback Write whenever you can, find supportive editors...

  18. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...designed a Golden Gate system based on the daily needs of an advanced molecular plant science lab.” Introducing...distinct sites where the enzyme will cut. You only need a single enzyme to cut fragments that are very different...
Showing: 421 - 440 of 830 results