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We narrowed to 879 results for: ids

Showing: 421 - 440 of 879 results

  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning.... Now, we've turned some of the most requested plasmids into lentiviral preps to speed up COVID-19 research... has now expanded to offer many of our control plasmids in new serotypes, as well as a new Cre dependent...

  5. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...this blog post for tips on using BLAST to verify plasmids. Analyzer guide A quick guide to all of ...browse the repository and quickly identify the plasmids and features you need for your next experiment...

  7. How Addgene Creates Content

    Type
    Blog Post
    ...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

  8. To Each HIS Own

    Type
    Blog Post
    ...stringent wash conditions.   Find polyhistidine tag plasmids here! Where should I place the tag and how many...polyhistidine tag is a string of histidine amino acids on the N- or C- terminus of a protein. Neither the...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

  9. Antibodies 101: Conjugation

    Type
    Blog Post
    ...antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene...

  10. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...create it. But while antibodies, as the OG (as the kids say) affinity reagents deserved to be celebrated...of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be used ...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...

  11. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...lightbulb or exchanging excitation filters). This avoids substantial changes in excitation power and will...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  13. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    .... Cells are cultured with isotope-labeled amino acids for several hours to several days, followed by a... a short period of growth with unlabeled amino acids. This washout period removes radio-labeled short-... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors...

  14. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...problematic because they require radiolabeled amino acids. The use of radioactive materials is highly regulated...

  17. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... that is something to recognize! Distributing plasmids, viral service, and more For the first time ...distribution to ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our ...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...ligation products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation result...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...

  19. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...

  20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...
Showing: 421 - 440 of 879 results