We narrowed to 470 results for: mag
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TypeBlog Post...coat! These are the things the rest of the world imagines when they think “scientist.” But leaving the bench...
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CRISPR-mediated Plant Base Editors
TypeBlog Post... Scholar at the Pennsylvania State University. Imagine you are dealing with a defective gene, Xm, the ... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... Host killing (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a... -
Plasmids 101: Codon usage bias
TypeBlog Post...transcription factor binding sites) As you might imagine, it’s not easy for humans to balance all of these... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020. Broad tropism: ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...receives stimulation in one of the two chambers (see image below). If a circuit is reinforcing, the animal ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution... -
Plasmids 101: Control Plasmids
TypeBlog Post...the protein. For example, fluorescence microscopy images resulting from our experiment that includes the... -
Fluorescence Titering Assay
TypeProtocol...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...introduce all desired codon changes (step 1 in the image above). A second universal primer is used to synthesize... -
Evolution of Lab Techniques
TypeBlog Post...restriction enzymes that cut DNA at specific sites (5). Imagine that you have a genome with unknown sequence. In... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...diseases, including dementia, gut disorders, nerve damage, and cardiac disease. This increased disease risk... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...For a simple example of using Gibson assembly, imagine that you want to insert your gene of interest into... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post... and the information is often confidential (see image below). Slide of information gathered by Postdoctoral... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...control option. Total protein stains or stain-free imaging systems are both effective for measuring the total... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...negatively selecting your cells of interest is magnetic bead-activated cell sorting, or MACS. But MACS... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post... the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...keeping, ELNs offer functionalities that are hard to imagine on paper, such as instant search through all saved... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post... recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered... -
Transferable Skills Guide: Time Management
TypeBlog Post...university. My to-do list might look something like the image on the right (and for those of you who can’t read...