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  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment...

  4. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...inverse orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    ...As a scientist, perhaps the most important part of your research is communicating the results of the ...the work. Yes, those control experiments are important - but without letting other scientists know the results...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...interest. See figure 1 below for details on the key components of RaPID. Ramanathan et al. also tweaked BirA...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...fragment, any additional recombination events are impossible even in the presence of Cre recombinase. This...in the absence of the endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most...

  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...quantified using qPCR primers targeting specific viral components such as LTRs, gag, WPRE, antibiotic resistance-genes...genomic DNA isolated, and primers targetting viral components or the transgene itself are used to quantify ...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...that the body’s architecture and plumbing are important. We started with learning the head is connected...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...polyclonal antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    ...editing application. And optimizing its sequence is important, as prime editing efficiency is affected by the...nucleotides. For longer templates, it is even more important to test different lengths and sequences, as unintended...

  18. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it...ThermoFisher, or Millipore Sigma. We’ll share the basic composition of these solutions below. Alternatively, companies...

  20. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too...you won't get as much out of it and it will be impossible to schedule. In the pre-event interviews, the...
Showing: 421 - 440 of 750 results