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We narrowed to 760 results for: mpo

Showing: 421 - 440 of 760 results

  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment...

  3. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...

  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...interest. See figure 1 below for details on the key components of RaPID. Ramanathan et al. also tweaked BirA...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    ...As a scientist, perhaps the most important part of your research is communicating the results of the ...the work. Yes, those control experiments are important — but without letting other scientists know the results...

  7. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...control of kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic ...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...considerations of the laboratory make object composition a smarter choice than inheritance. Some containers...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

  10. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...inverse orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...that the body’s architecture and plumbing are important. We started with learning the head is connected...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

  16. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...polyclonal antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    ...editing application. And optimizing its sequence is important, as prime editing efficiency is affected by the...nucleotides. For longer templates, it is even more important to test different lengths and sequences, as unintended...

  19. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it...ThermoFisher, or Millipore Sigma. We’ll share the basic composition of these solutions below. Alternatively, companies...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...
Showing: 421 - 440 of 760 results