...thresholds for validating CRISPR experiments, many are turning to next-generation sequencing options over older...configuration, produces many false negatives Combining sequencing techniques can ensure validated experiments...Editing Specificity” by Tycko et al. 2016 and “Defining and improving the genome-wide specificities of.... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities of...

...Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its Fruit Is Sweet...types not within the vicinity of the injection. Examining serotypes 1, 8 and 9, not only do they undergo...transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course, one cannot...at the University of Manitoba. Her project is examining the use of DREADDs (Designer Receptors Exclusively...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...

...articles in less-prestigious journals have been gaining ground. A study issued by Google in October entitled...report also noted, papers detailing Nobel Prize-winning research typically weren’t among the most cited...impact.” As we look ahead to the end of a year and beginning of another, perhaps it’s worth reflecting on a...Other Novel Measures for Scientific Impact" from Opening Science - The Book Bollen J, et al. "A Principal...

...countries. We, like everyone around the world, are learning to navigate the changes brought about by the pandemic.... As always, we seek to understand our role in opening up science to help create new knowledge and research...opportunities. I have spent the last 9 months quietly learning about Addgene’s past and present, but the real...place in it. This period of careful reflection and planning will allow us to continue to serve the scientific...

...providing cloning grade DNA to you, we'd save you days of time so that you can begin your cloning experiment...this year were influenced by COVID-19 and our new cloning grade DNA service. We've had 11 blog posts on COVID... and industry. This year, we also launched the cloning grade DNA service. This service features 210 plasmids...

...was fortunate to see the event planning unfold from the very beginning. The board picked a theme (Bio-...DayCon DayCon and other similar events show how meaningful science outreach can be, both for scientists ...communication and organizational skills provide great training for a number of alternative careers in science...

...the hybridoma can occur, known as genetic drift, meaning that over time, the antibody you expect it to produce... the advantage of being significantly smaller, meaning they can recognize epitopes that full antibodies... detect the presence of proteins from samples containing a mixture of proteins. The proteins are separated...antibodies to detect proteins is referred to as staining. This panel of antibodies is used to both identify...enzymatic reaction. Figure 5: Immunofluorescence staining for CD31 (green), CARD14 (red), and pNFkB (blue...

...Chapter 10: Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional... topics discussed during the interview. Happy listening! Listen to the interview with Science in the News...communicating science to the general public and training the next generation of science communicators. ...

... She suggests to pick up hobbies (for her its gardening) that help you relax and enjoy life. “I love academics... the afternoon during the child-rearing years, learning “lots of interesting things from children, their... cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...Advice for Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project...

...scientists. It takes us a decade or more for just the “training” portion of our careers. In that time we may have...organizing mentoring programs for scientists and doing training to help mentors and mentees have fulfilling, productive...these things and more. It is instructive (and entertaining) to review this list of bad mentor behaviors...openly Gives honest feedback without ever being demeaning Does not micromanage (teaches you to fish, doesn...

...surprisingly simple, requiring only a PCR product containing the modifying sequence flanked by approximately...distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease sites...Formosa has deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of...additional benefit of multiple selection markers for combining modifications within a single strain. In addition...

...conventional microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...multiplexed tissue imaging method that uses iterative staining and chemical bleaching to allow for high-resolution... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...are the three main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector...defined neuronal populations using viral strategies Combining different approaches can increase the specificity...information. To complement these efforts, we’re beginning to share data through our recently launched AAV...

...connections with coworkers and doing something meaningful with my career. I can’t pinpoint exactly when... mine is mainly within content and business.  Joining the Addgene crew!  This pattern was consistent:...bigger projects I was tasked with was creating and sustaining a biweekly report of our social media analytics...free time, she enjoys reading, petting dogs, and winning the office Just Dance competition.    Special ...

...in Drosophila. SPARC was developed by Thomas Clandinin’s lab, and incorporates several common genetic ...both for visualization and for optogenetics. The Clandinin lab used SPARC to image the calcium response in... YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin TR (2019) SPARC: a method to genetically manipulate...resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more...

... hormone,” it’s also involved in reinforcement learning, decision making, and motor control.  What do... mice Report modulation of DA signaling during learning and reward consumption in live mice Find dLight...Drosophila Detect DA dynamics in response to a threatening looming stimulus in vivo in transgenic zebrafish...release in live mice over the course of Pavlovian conditioning Find the GRABDA sensor here! Overall these ...

...keep reading). Corning takes back and recycles a lot of their plastic packaging (from Corning®, Falcon®, ...plastics are ever recycled, so adopting a few meaningful changes in the lab could make a big difference...person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your...

...away-from-the-bench career option. So if you’re interested in learning more about science communication careers, you’...s paid off. Work is never boring. “I just keep learning on the job. It keeps it interesting.” And while...accomplishment.” He started working on them shortly after joining IDT when his boss was looking for someone to take...never get off of the ground. So limiting that, and defining a couple of key terms is a better approach. Also...

...make us self-sustaining. With this unique model, we are able to spend resources on maintaining quality and...investors or stock. All of our proceeds go into running the repository and serving scientists better. That... this trust by keeping excellent records and maintaining confidentiality where it is appropriate. This...

...when you’re running your next AAV experiment, use these questions as a guide for designing your future...Data Hub to quickly find details they need for planning their next experiments using a specific AAV tool...activate receptors? If you’re in the process of designing your experiment, read our blog post on important...