We narrowed to 538 results for: sequence

1. With Our New Viral Service, We're Taking CRISPR Further!

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   ...must amplify the library, use next-generation sequencing to ensure that the library has retained gRNA ...

2. Targeted Mutagenesis with EvolvR

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   ...use to tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate...

3. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   ...anchoring domains – GPI and SGZ. Fig: 4 Sequence schematics for PDGFR, GPI, and SGZ display constructs...

4. Behind the scenes: Addgene’s new search engine and more

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   ... data our depositors share and enrich it with sequencing data; as a result, the materials in our catalog...

5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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   ... string of amino acids that preserves the sequence of your gene and of the fusion protein. Read more...backbone for cloning and expression of new shRNA sequences Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone...

6. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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   ...  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends: Sequence based input is by far the...

7. Worm Expression Resources

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   ...worm ( C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene...

8. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...be programmed to bind tightly to arbitrary DNA sequences and have been shown to achieve ~100 fold repression...

9. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...into the genome randomly, while others use attP sequence docking sites so that you can control exactly ...

10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...collection reported to date, containing all 620 sequences of probable mature human miRNAs. Weiss’s collection...

11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...caspase) cleavage site (DEVD). Upon cleavage of the sequence, β10 and β11 can rearrange to their normal antiparallel...

12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ... mVenus linked to a mitochondrial localization sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted...

13. Viral Vectors 101: Viral Vector Elements

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    ...terminator regions and are slightly different in sequence. Both LTRs do share common repeat regions in addition...

14. Hot Plasmids - February 2022

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    ...recombination by finding acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas ...

15. Antibodies 101: Isotypes

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    ...expressing a different isotype, the intervening DNA sequence is deleted, which places the variable region next...

16. Antibodies 101: Polyclonal Antibodies

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    ...generate antibodies against, or a unique peptide sequence derived from this protein. At the same time, an...

17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...editors capable of editing previously uneditable sequences. Four DNA base swaps, so called point mutations...

18. Allen Institute for Cell Science Plasmid Collection

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    ...data and images from the hiPS cell lines gRNA sequences used in the creation of the hiPS cell lines The...

19. Deep Dive: qPCR

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    ...product on an agarose gel, then cutting out and sequencing the band for verification. Primer dimers will...uMelt is an online tool that uses the  amplicon sequence and thermodynamic and experimental parameters ...

20. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ... the dCas9-CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the...photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a double...