We narrowed to 470 results for: Lif

1. Plasmids 101: The Promoter Region – Let's Go!

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   ...expression mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, ...

2. CRISPR 101: Validating Your Genome Edit

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   ...   How to perform a TIDE First, use PCR to amplify the target region of interest. Then Sanger sequence...

3. Transferable Skills Guide: Conflict Resolution

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   ... be the most controversial, so I would like to qualify it. If you are attempting to resolve conflicts ...

4. Antibodies 101: Introduction to Immunofluorescence

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   ...bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness. Generating...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template with a “sloppy” version...

7. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...blot, and depending too much on normalization can amplify other errors in experimental design and gel quantification...

8. Antibodies 101: Introduction to Gating in Flow Cytometry

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   .... When he's not in the lab, Paul enjoys weight lifting, sailing, and reading.   More resources on the...

9. 10 Steps to a Perfect Science Talk

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   ...talks We are not talking about lab meeting here. Lifeless presentation of experimental results in the order...

10. CRISPR 101: Homology Directed Repair

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    ... a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed. Regulation of DNA double-strand...

11. Evolution of Lab Techniques

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    ...interest by recording DNA abundance throughout the amplification process, rather than just at the end as in standard...

12. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ... last, and more importantly, if it does extend lifespan. This work should be done in mouse and large animal...

13. Plasmids 101: Common Lab E. coli Strains

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    ...vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96...

14. Antibodies 101: The Basics of Western Blotting

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    ...antibody molecules bind the primary antibody and amplify the detection signal.   Analyzing the western blot...

15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...feature (e.g., a fluorescent protein), you can amplify the desired region using primers containing the...

16. Viral Vectors 101: Optogenetic Tools

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    ... pathways. Additionally, as GPCR signaling is amplified in successive steps along a pathway, there can...

17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Arandel, L., Marhic, A., Chapart, M., Vasseur, S., Benkhelifa-Ziyyat, S., Bigot, A., Butler-Browne, G., Mouly...

18. PiggyBac-ing Through the Genome Editing Field

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    ...between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional...

19. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001...

20. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ... inserts can be made by PCR using primers that amplify the DNA sequence of interest and flank it with ...