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Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Learn more about the common E. coli strains used in the lab.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Addgenie Emma Markham describes her winding career path and provides advice on how you can find a great job after undergrad.

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and explain common applications.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.

Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems.

Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses.

Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses.

Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.

Find a list of universal sequencing primers and other common sequencing primers.