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We narrowed to 993 results for: Des

Showing: 441 - 460 of 993 results

  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...pseudotyping allows these viruses to infect neurons. Besides altering the host tropism of your virus, there ...neurons. However, VSV-G’s broad tropism can be undesired for in vivo studies since there’s the potential...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    .... After months, or years, of grueling effort to design, fund, and conduct a research project, the vast...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract genre...websites). WeShareScience is a social platform that provides links to the videos, keeping the copyright and...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...client might need help with the intricate task of designing a strategic vision for a portfolio of candidate...content-rich, easy-to-understand set of PowerPoint slides. What are some of the more challenging parts of...enjoy the challenges of healthcare consulting for decades, others go on to work with commercial teams at ...nature of the job and the ways to prepare for it. Besides those conversations, reading online healthcare ...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to a ... you should also be ready to test three or four guides directed towards that gene as there can be a large...transcription. That being said, we generally pick guides by putting the promoter of the gene into a gRNA...

  5. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...the time of manuscript submission. The rationale includes increasing the ability of reviewers (and, once...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...could be mitigated by on-line services such as SlideShare, Figshare and Dataverse. Just as Addgene helps... raise the alpha isoform of GSK-3 to the same (deserved) stature as the beta isoform. Follow him on Twitter...

  6. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require...or search the tables below for CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...pathological developments (Vieira et al., 2019) Besides their ability to regenerate, the axolotl has several...scientific history, highly characterized genome, and widespread utilization, Arabidopsis has made significant...Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research development. Arabidopsis...allows for easy cloning of constructs specifically designed for use in Arabidopsis. There are also important...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...same fluorescent markers, Brainbow 3.1 and 3.2 (described in the same publication) are unique because by...addition to the XFP in the construct, dBrainbow includes small epitopes fused to each XFP. Secondary antibodies...introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of ...cell type. The Confetti mouse was first used to describe the self-renewal of cells in the intestinal crypt...

  9. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...blot: we meet again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization...normalization can amplify other errors in experimental design and gel quantification. Figure 3: In a perfect...final blot. Quantifying the entire lane of protein provides a loading control that can be used for normalization...Antibodies 101: The Basics of Western Blotting Technical Design of a Western Blot Troubleshooting and Optimizing...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Applying for a Faculty Position: the View from Both Sides," are summarized below. Check Out Erik Snapp's ...You want people to focus on you and your ideas. Slides will be     distracting. What is the big question...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...single malt whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group...

  11. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...cofactor binding proteins as described above. The smallest tags are peptides that are engineered to bind...homology domains can be used to detect specific phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production...Péter, and Tamas Balla. "Live cell imaging of phosphoinositides with expressed inositide binding protein domains...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...expression to specific tissues and cell types. If desired, spatiotemporal control of transgene expression...systemic AAVs to find commonly used vector cargo designs and administration doses. Note: If capsid immunogenicity... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology... prioritization for cell type-specific enhancer design. eLife,11, e69571. https://doi.org/10.7554/eLife...

  13. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...easy to implement due to the simplicity of gRNA design, programmable for different genomic loci, capable...or search the tables below for CRISPR plasmids designed for the visualization of genomic loci. To learn... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  14. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

  15. Optogenetics Plasmid Collection

    Type
    Collection
    ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for... "transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...

  16. TALEN Engineering

    Type
    Collection
    ...laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...

  17. Resolute Plasmid Collection

    Type
    Collection
    ...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes. One consists of NLuc fused...Reporters. The LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection...BRET probe for imaging. These authors set out to design a better fluorescent protein that could be co-imaged...acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...every item I tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab...industry positions. There were, however, some downsides to working in a large commercial company. In my...aspects of the company and gave me the freedom to design new projects and grow in the role. I understand... job different every day. I love the freedom to design and develop my own projects, but working remotely...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the...or search the tables below for CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, ... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...
Showing: 441 - 460 of 993 results