We narrowed to 672 results for: FIE

1. Stepping into a Virtual Internship: My Experience at Addgene

   Type

   Blog Post

   ...what it was like to work at Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke...

2. What's New in CRISPR - December 2019

   Type

   Blog Post

   ...this system was optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit...

3. Editor's Choice, August 2016

   Type

   Blog Post

   ...deaminase to make the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues...

4. Modulate the Activity of 17 Signaling Pathways with One Kit!

   Type

   Blog Post

   ...apoptosis. For each of these 17 pathways, they identified a set of one to three mutant complementary DNAs...

5. Management for Scientists: Seeking Feedback

   Type

   Blog Post

   ... coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful teams...

6. Scientist Networking: What is an Informational Interview?

   Type

   Blog Post

   ...meeting with people working in diverse and unusual fields. My favorite final trick is to end each Informational...

7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose...

8. SciComm with the Experts at Science in the News Part 2

   Type

   Blog Post

   ...SITN team brings in scientists from a variety of fields as well as students from the ed school, design ...

9. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   ...had turned red. They subsequently confirmed that purified GFP could be imaged in the green state after excitation...

10. Science as Art

    Type

    Blog Post

    ...etc. Photography is critically important in many fields of biology and I believe it could have made the...

11. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type

    Blog Post

    .... Biallelically disrupted cells can be easily identified. Even in the case of clones with monoallelic ...

12. Inside Addgene's 10 Year Anniversary Gala

    Type

    Blog Post

    ...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...

13. Addgene is Expanding Our Viral Vector Service!

    Type

    Blog Post

    ...produced, the request will be denied, and you will be notified via email. There is no charge for the request ...

14. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type

    Blog Post

    ...can be used with many conventional microscopes, amplified staining methods, and oligonucleotide-based staining...

15. Working 9-5: an Addgene Co-op

    Type

    Blog Post

    ...majority spend two to three) working in your chosen field. This cooperative education experience (co-op) is...

16. 6 Steps to Submitting a Resume That Gets Seen

    Type

    Blog Post

    ...resumes The cover letter must address how you are qualified and why you are interested specifically in the...

17. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type

    Blog Post

    ...their labs studying the mechanisms of RNAi, the field for which Craig Mello and Andrew Fire won the Nobel...

18. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type

    Blog Post

    ...affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed...

19. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type

    Blog Post

    ...go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists from...

20. Protocol - Bacterial Transformation

    Type

    Protocol

    ...the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane...