We narrowed to 472 results for: FLU;
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TypeBlog Post... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...
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Antibodies 101: The Basics of Western Blotting
TypeBlog Post...composed of a nitrocellulose or polyvinylidene difluoride (PVDF) in a step called protein transfer. While... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...collectively little about how they infect and influence the evolution of their bacterial prey. Currently... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...on their own, into the rough waters of a world in flux. The professional development and networking resources... -
Tetracycline Inducible Expression
TypeCollection... shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target... -
9 tips for a successful postdoctoral experience
TypeBlog Post...in the Endoplasmic Reticulum, optimization of fluorescent proteins, and live cell imaging approaches. ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...such as drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant... -
Finding Your Science Policy Path
TypeBlog Post...outside of the government itself. You can work to influence Big P policy from a post in non-governmental organizations... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post... I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low... -
Antibodies 101: Validation
TypeBlog Post...aware that overexpression of a protein can also influence antibody binding by altering the relative concentration... -
Biosensor AAV Preps
TypeCollection...Service: Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...tagging in human cells Plasmids 101: Protein Tags Fluorescent tagging of endogenous genes with SapTrap Resources... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post.... A tumor’s genetic composition is constantly in flux and is dependent on the selective pressures placed... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post.... Many plasmid elements such as promoters or fluorophores are interchangeable across organisms. When using... -
Plan Your Experiment
TypeCollection... selectable marker (antibiotic resistance or fluorophore) are also relevant. Delivery Using Plasmids The... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...You must also be able show the impact of your influence on your field of employment. This fact is best... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. These vectors contain... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post... as well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements... -
Neurodegeneration Plasmid Collection
TypeCollection...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated... -
Antibody Guide
TypeGuide... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...cell or tissue sample. Figure 6: Immunofluorescence, in which fluorophore-conjugated primary antibodies ...label cells in a sample. Immunofluorescence (IF) This method uses fluorophore-conjugated antibodies to...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...