...protein that no other protein in the expression system has. This is where affinity tags come in – they are peptides...solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...

...scheme, known as the Baltimore replication classes, has been developed around how they replicate (Figure ..., which retains the protein coat (and envelope), has a “gutted” genome with genes central to the replication...

...sciences research. The rare disease research community has certainly noticed: This year, the BeHEARD (Helping...McSweeney (right), a graduate student at Duke who has recently moved to Columbia University with David ...

... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ...

...enables you to discuss a person's performance or what has been learned, it helps to plan effective next steps...about areas they need to improve in. If a manager has concerns about an employee and the employee doesn...

...share reagents so freely with the academic community has clearly made a huge impact on this field. Advances...talks, I oscillated between amazement at how much has been done with CRISPR technology in just 2 years ...

...an organization such as COMPASS. Although COMPASS has a special interest in ocean science, they have good...Program at the University of California, Santa Cruz, has been turning scientists into confident and connected...

...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...

...trend in funding and space in academic research labs has more students looking for other options. While graduate... skills for alternative careers. Science Careers has developed a web-based, career-planning platform called...

...learn about antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies...

...increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed probe sets that...high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly screen buffer conditions for... 200 mM KCl at pH 8.5. This optimization method has been used internally at NEB to screen additional ...additional ligases, conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase....

...measured using visible light scatter The optical system has both visible and fluorescent light sources. Visible...autofluorescence from your target cell type or that has arisen from fixation. Also include an isotype control...secondary antibody control in which the primary antibody has been omitted from the staining protocol. This control...gating during data analysis.  We hope that this blog has provided you with an overview of flow cytometry for...

...CRISPR/Cas editing toolbox for C. elegans Recent work has further improved the efficiency of genome editing... (Farboud et al., 2019). Work from the Mello lab has dramatically improved RNP editing with linear repair...elegans (Ebbing et al., 2017). Because C. elegans has an AT-rich genome, it can be difficult to find an...whereas import of CRISPR technology into C. elegans has been a transformative innovation for our community...

..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...

...the opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well ...scientists who pioneered the development of optogenetics, has genetically manipulated a number of the opsin genes...kinetics of opsin activity/inactivity. Dr. Deisseroth has also generously made many of his optogenetic constructs...stereotaxic surgery (see videos below). The Deisseroth lab has developed many of these AAV constructs with various...

...RNA and can express fluorescent proteins. This lab has created an automated tank for feeding and maintenance...scolopes (Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually... injection (hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis...

... improve its stability in vivo. However, Spinach has proven useful in the engineering of riboswitches....syndrome (Strack et al., 2013). Since then, Spinach2 has been used to investigate intracellular viral DNA ...better choice for imaging than the monomer. Broccoli has been developed into a split aptamer that can be used...

...stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires...Once the average copy number for a given plasmid has been reached, negative feedback circuits reduce replication...

...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...