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We narrowed to 856 results for: HA

Showing: 441 - 460 of 856 results

  1. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...available, the next best thing is a protein that has been validated for an assay that uses similar sample...Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western because...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...protein that no other protein in the expression system has. This is where affinity tags come in – they are peptides...solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...

  5. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...scheme, known as the Baltimore replication classes, has been developed around how they replicate (Figure ..., which retains the protein coat (and envelope), has a “gutted” genome with genes central to the replication...

  8. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Avengers This year Addgene, where my wife Lianna has made her career as Senior Director of Biology, is...pre-clinical development for First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy...

  9. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg. Plasmid Serotype Name...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...overlap extension PCR. Although each type of cloning has its advantages, most scientists still encounter several...science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning...

  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ...

  13. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...enables you to discuss a person's performance or what has been learned, it helps to plan effective next steps...about areas they need to improve in. If a manager has concerns about an employee and the employee doesn...

  15. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...an organization such as COMPASS. Although COMPASS has a special interest in ocean science, they have good...Program at the University of California, Santa Cruz, has been turning scientists into confident and connected...

  16. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...

  17. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...trend in funding and space in academic research labs has more students looking for other options. While graduate... skills for alternative careers. Science Careers has developed a web-based, career-planning platform called...

  18. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...learn about antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...measured using visible light scatter The optical system has both visible and fluorescent light sources. Visible...autofluorescence from your target cell type or that has arisen from fixation. Also include an isotype control...secondary antibody control in which the primary antibody has been omitted from the staining protocol. This control...gating during data analysis.  We hope that this blog has provided you with an overview of flow cytometry for...

  20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...CRISPR/Cas editing toolbox for C. elegans Recent work has further improved the efficiency of genome editing... (Farboud et al., 2019). Work from the Mello lab has dramatically improved RNP editing with linear repair...elegans (Ebbing et al., 2017). Because C. elegans has an AT-rich genome, it can be difficult to find an...whereas import of CRISPR technology into C. elegans has been a transformative innovation for our community...
Showing: 441 - 460 of 856 results