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We narrowed to 771 results for: LIS

Showing: 441 - 460 of 771 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...

  7. March for Science

    Type
    Blog Post
    ...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for ...like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...new empty backbones By Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's...the payload sequence into the genome. The team established that they could reprogram the genomic target ...

  14. A Control for All Seasons

    Type
    Blog Post
    ....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...duration of only three months and made a good job to list on my CV. Three months was a perfect duration for...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...

  20. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...
Showing: 441 - 460 of 771 results