We narrowed to 526 results for: MEA;

1. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   ...for more information about this feature!).  This means you can see which labs have already used a particular...

2. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   ...process. While CRISPR is certainly not the first means to carry out so-called “forward genetic screening...

3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   ...biosafety officer should be familiar with appropriate measures. Barriers should be a component of all gene drive...

4. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ... not filter low complexity regions by default, meaning that you do not have to remember to adjust any ...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   ...? Zeocin is genotoxic when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA...

6. An Introduction to Adenovirus

   Type

   Blog Post

   ...synchronized exposure under these conditions once the measures were lifted (Servellita et al., 2023).  This anecdote...

7. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   ...worthwhile to generalize the idea of “transduction” to mean any transfer of material from the virus to the host...

8. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...also too large to be expressed in bacteria, which means their production requires animal sacrifice and precludes...

9. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   ... you know how to do the job. You may think this means you’re guaranteed to get the job, however, many ...

10. MXS Chaining

    Type

    Blog Post

    ...MXS-chaining also has the advantage of being directional, meaning that (in the previous example) one can control ...

11. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...In our experience, good amplification after PCR means successful plasmid assembly (if primer design is...

12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ...CRISPRko results in a complete loss of function meaning that if an essential gene plays a role in the desired...

13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    ...RNP method provides scientists with an additional means to deliver CRISPR components to their experimental...

14. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...essentialist links between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally...

15. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb;15(2):78-82...

16. Plasmids 101: Colony PCR

    Type

    Blog Post

    ...because you get the expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure...

17. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...tubes. 1D barcodes are better for tubes that are meant to be read one at a time. 1D barcodes can be purchased...

18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...outside of our break room. A ring of the chimes means something fun is going on and to get there fast!...

19. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...to help you enter and to have a successful and meaningful career in the grants profession: 1. Build on ...

20. Plasmids 101: Protein tags

    Type

    Blog Post

    ... a commercially available antibody, that doesn’t mean it’s a good one), then your first step towards detecting...