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We narrowed to 840 results for: Met

Showing: 441 - 460 of 840 results
  1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post
    ...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ... al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated...
  2. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a .... And, naturally, rarely are the optimization parameters the same for all three components, meaning that...Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural Amino...
  3. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...understand how the brain works. There are two methods of neuronal tracing (Fig. 1): one to visualize ...catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies...neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon is...
  4. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post
    ...transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies ... hundreds of transcription factors. We wanted something that would allow automation. This design cuts ...pressure on many other labs that may be doing something similar. There may be other variants of this that...
  5. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post
    ...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...models, and software tools in the Materials and methods section of their manuscript to make resources easier...information you provide on data, materials, and methods, the more valuable your paper will be to the scientific...
  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have ...to examine the effect of a gene on cholesterol metabolism. The TALEN kit is quite useful in generating ...
  7. Antibodies 101: Fab Fragments

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    Blog Post
    ...super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...
  8. Antibodies 101: Affinity Reagents

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    Blog Post
    ...diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies...including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be used either with or...
  9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ...visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or...techniques. Thus, these proteins provide a versatile method to identify the expression of transgenes of interest...Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128...
  10. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post
    ...enzymes that you are using are not blocked by methylation like XbaI, ClaI, etc. If you are doing multiple...to be strategy specific, there are few general parameters that you can adjust: temperature and time of ...molar ratio of 1:2 (plasmid:insert), but this parameter has to be optimized according to the strategy ...
  11. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...pharmacological or genetic approaches, but only a few such methods exist and they only work for a subset of kinases...kinases. The Problem:  There is no common method for creating optically controlled kinases. The Solution:...KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating other signaling pathways...
  12. CRISPRainbow and Genome Visualization

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    Blog Post
    ...efficiency was similar to that of a comparable FISH method. Importantly, they did not observe labeling when...with both intronic and exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene...genome editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed...
  13. Transferable Skills Guide: Public Speaking

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    Blog Post
    ...panicked silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in...options were to stop talking until I could present something coherent or to quickly string together all the... above to make a strategy that can help. Even something as simple as telling yourself, “I can stop and...
  14. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post
    ...been “working on new experimental and theoretical methods to push the scale of genetic engineering, with ...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...
  15. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post
    ...coaching plans that are financially accessible. Sometimes only a few hours of coaching time can make an ...Coaching. One nice thing about a group is that sometimes this offers the option of matching with the best...a good match as a coach for many scientists.  Sometimes it can be helpful having a coach that can offer...
  16. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post
    ...there are for off-target edits to occur. Although methods to switch on Cas activity using light or drugs ... Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi....natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020...
  17. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ...minute! Fun fact! Electroporation is one popular method scientists use to promote transformation, but it...offer resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive ...inducible expression systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing...
  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ... this blog post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols...Andrea Pauli and Julius Brennecke. They developed a method that uses loop-mediated isothermal amplification... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...
Showing: 441 - 460 of 840 results