... in Lower Geyser Basin. They used a mini-metagenomics method using microfluidic parallelization to sequence... collection. Figure 1: Paul included a voltmeter on top of the battery box to monitor charge status...

...viral production strategies or developing novel methods for data analysis, our team’s goal is to provide...and laboratories alike as an easy and inexpensive method to improve AAV yields. Although we only investigated...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...research. Long story short, telling a good story is something that can be learned, and will serve you well in...yourself jumping backwards in time to explain something, rethink your structure. Develop your protagonist...but there are plenty of other options. Choose something with emotional significance that is likely to ...

...the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...

...light. In a recent article published in Nature Methods, Boyden and his team describe the discovery of ... tree of life. Our most recent paper in Nature Methods offers the first true two-color pair. We'll be ...Neural Populations. Nathan Klapoetke et al. Nature Methods. 2014 Feb 9. (PubMed). Read More: Other Optogenetics...

... more effective mAb therapeutics. The in silico method of immunogenicity assessment can help in determining...All approaches have their own limitations. Using methods together can inform decisions about in vivo application...for Sequences Containing Unnatural Amino Acids: A Method Using Existing in silico Algorithm Infrastructure...

...rate that study as untrustworthy, inaccurate, methodologically flawed, and biased. As a result, the control...because a greater familiarity with scientific methodology makes it easier to spot even minor weakness or...might struggle to understand. Why should we trust something that we cannot read or verify for ourselves? Further...

...information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples on dry...following transport or storage on dry ice. Nat Methods. 2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2...recombinant adeno-associated virus vectors. Mol Ther Methods Clin Dev. 2014 Aug 13;1:14034. PubMed PMID: 26015974...

...exported as a picture. I believe that showing something different from time to time breaks up the monologue...monologue a little and gives participants something else to focus on. Use collaborative cloud apps It is... Take brain breaks Meetings can get very long sometimes, and interaction fades away the longer we are ...

... biosensors, which are optimized for specific parameters: jGCaMP7s is the most sensitive responder to...primary inhibitory neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal...Optogenetics Optogenetics continues to be a useful method for manipulating specific neurons. We recently ...

...support your favorite local exercise spot or find something new. We usually have yoga class twice a week at...more independently. For example, they could do something like puzzling or crafts, while listening to podcasts...registered report, where peer review is based on the methods, not the results. Also consider using this framework...

...this Plasmid” section. Having IDs linked to these metadata and publications help to enable reproducibility...cite any Addgene plasmid in your materials and methods section have been updated to include the option...how to cite using the PIDs in the material and methods section of your publication. You should carefully...

... et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision. Our...or double stranded donor DNA with other design parameters. Example single stranded donor DNA and a presumptive...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

...probe and build new systems. Such goals require methods to directly activate and inactivate biological ...that prints a chemical image, as well as a genetic method for the computational problem of edge detection...identified in bacteria, and with similar optimization methods, many of these systems could be made suitable for...

... reporter occurs slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein...Gaussia princeps) 480 20 Coelenterazine None Yes Metridia longa 480 24 Coelenterazine None Yes North American... its activity to produce light, often with a luminometer or scintillation counter, and the activity measurement...

... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a .... And, naturally, rarely are the optimization parameters the same for all three components, meaning that...Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural Amino...

...understand how the brain works. There are two methods of neuronal tracing (Fig. 1): one to visualize ...catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies...neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon is...

...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... depending on your interests. You can look at something as basic as survival. For those interested in ...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

...both the biological system as well as the imaging method, we recommend basing your selection on a small-...is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the...practical brightness takes all application specific parameters into account, including the specs of your microscope...

...protein structure. Sometimes this provides a great deal of clarity. But, often these methods don’t agree. Confusing... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ...