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We narrowed to 885 results for: NSI

Showing: 441 - 460 of 885 results

  1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... this type of ELISA is extremely specific and sensitive to the target protein due to the use of multiple...concentrations of your protein of interest. The optical density (OD) - the measure of light absorbed by a solution...any experimental wells in that range of optical densities. If your standard curve does not have a linear...

  2. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...article we will discuss general viral safety considerations and best practices for handling lab-grown viruses...replicate and continue the infection cycle once inside the host. While the virus can still enter (and ...into the host’s DNA. These viruses also have a propensity to integrate into proto-oncogenes, which can ...

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...fluorescent in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...requires a camelid animal facility which can be expensive to gain access to routinely, and it can be especially...the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ribosome,...constructed, they are used to produce a phage library consisting of phages that display the candidate sybodies...

  6. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...poster – now it’s time to practice your delivery. Consider your audience: Who will be attending the session... for Cancer Research Annual Meeting, you might consider including more background on how your virus of...practice: Rehearse your presentation with people inside and outside your field. Your colleagues, lab mates...

  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...If your poster material is nearing publication, consider uploading your work to a preprint server (and ... of the things we've mentioned above could be considered networking, but here are some more general tips...resume and prepare business cards. You might not consider being a student or postdoc a “business,” but having...

  8. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...batch analysis because of its ease-of-use and comprehensive reports. With CrispyCrunch, you get the all ...summarisation score".) For CrispyCrunch, we wanted to consider both factors, as a bench scientist would looking... our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find other CRISPR software...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...carry out a high-throughput genetic screen, it is expensive and time consuming to individually generate gRNAs...Most insertions can only be detected by labor-intensive processes like PCR or by evaluating visual phenotypes...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  10. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...vivo HSC expansion cultures. A) Experimental design. B) UMAP of cell states in ex vivo expansion cultures...fluorescent proteins just haven’t met your needs, consider trying mGold2s or mGold2t for your next experiments...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis...equipment. Hopefully this post has provided some insight on these differences in viral production and will...versus insect cells for viral production Consideration Mammalian cells Insect cells Viruses AAV and...

  12. Pooled Library Amplifications

    Type
    Blog Post
    ...and how will you ensure this?). You should also consider if there are any features of the library backbone...difficult to transform.    Figure 1: Factors to consider when creating a pooled library amplification protocol...BioRender.com.   The most important factor to consider is your desired library coverage, since this will...

  13. PhD Applications After COVID

    Type
    Blog Post
    ...in-person events change seemingly daily (and are inconsistent between universities!) This guide tells you ...to be incredibly useful, as it gives a more comprehensive idea of that advisor’s research interests which...program and faculty. Here are some questions to consider for your online meetings:  Is this department...

  15. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...only help. Like any major life decision, the transition felt scary. While I knew two people from graduate...an industry postdoc is something that you’re considering. Like anything, there are pros and cons to postdocs... to enter academia, it is easier to make that transition from an academic setting—especially if job applications...

  16. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...result in appropriate levels of colony plating densities. Ligase fidelity and assembly efficiency Five...possibility. Recently, NEB researchers profiled the comprehensive fidelity of cohesive end ligation by this enzyme..."A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...capsids were packed with a GFP transgene and GFP intensity and transduction efficiency of neurons were measured...AAV.PHP.eB and AAV.PHP.S had 1.5- to 2-fold higher GFP intensity compared to the strains they were derived from...Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about using AAV ...

  18. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...of looking for The One Method to Rule Them All, consider the following four questions to figure out which...than it is to design one demonstrating mitosis.   Consider also the amount and type of information you want...competitive while digital can be oversaturated. Transition from academic to informal writing can difficult...

  19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...So… why do some people still use amp? Carb is expensive (relative to amp), and for some applications, ...with Amp, timesaving for transformations. Con: Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside...Like carb, spectinomycin is the more stable and expensive antibiotic compared to streptomycin. Spectinomycin...

  20. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...inhibit cell cycle regulatory proteins responsible for the transition out of S phase, so cells stay in an...exogenous DNA can be used as well. Below are some considerations for designing donor DNA for successful HDR ...
Showing: 441 - 460 of 885 results