We narrowed to 532 results for: PAN

1. Hot Plasmids - October 2020

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   Blog Post

   ...structure and function, with the advent of each tool expanding our ability to study and understand the complex...

2. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ... innovative methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum...

3. Antibodies 101: Stripping and Reprobing Western Blots

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   ...composition of these solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure...

4. Plasmids 101: Modular Cloning

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   ...Scripps Research, where he works on genetic code expansion.  References and Resources References Weber E...

5. Antibodies 101: Choosing the Right Isotype

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   ...Secondary antibody selections for a four-antibody panel, staining in parallel    In short, when staining...

6. Tips for Titering Your Lentiviral Preps

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   ...on expression of a surface marker, subcloning, expansion, and confirmation. This procedure can take several...

7. The Materials Science of Optogenetics Experiments

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   Blog Post

   ...required to produce your fiber optic implant. Companies such as Thor Labs and Precision Fiber Products...

8. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   ...at my workshops, I get nods from nearly every participant. Robert Bramson, author “Coping with Difficult...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ... analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab demonstrated they could use the...

10. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model bacteria including pathogenic...

11. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    ...takes 4-7 days, not counting the time needed to expand the HEK293 cells. Check out the Addgene AAV Production...

12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    ...get yourself a copy of Fly Pushing by Ralph J. Greenspan. It’s a fantastic book for newbies on how to work...

13. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas...

14. Magnetic Control of Proteins: More than a Dream

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    ... Purohit, A. M., Grippo, R. M., Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel, M...

15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...off-target editing rates. This PAM flexibility should expand applications that require very specific targeting...

16. Transferable Skills Guide: Managing a Team

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    Blog Post

    ...the next project, reaching out to other teams, expanding your network…. I found it helpful to plan my week...

17. 3D Printing Meets CRISPR Cas9

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    ...using 3D printing technology. The tagline for our company is “…where molecules become real”.  We sometimes...

18. Experimenting with New Careers while in Grad School

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    ...promote CINSO events and recruit speakers and participants for our professional development programs. I...

19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ...is there is a paucity of resources. We wanted to expand the toolkit. We have included a wider range of ...

20. Which Fluorescent Protein Should I Use?

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    ...Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent proteins as biomarkers...