We narrowed to 589 results for: RET

1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

2. Targeted Mutagenesis with EvolvR

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   Blog Post

   ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

3. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ..., does not require standard curves, and can theoretically detect a single target molecule in a sample....

4. Plasmids 101: Screens vs. Selections

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   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

6. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

7. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   Blog Post

   ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

8. To Each HIS Own

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   Blog Post

   ... specific proteins within a system. This can be pretty complicated given that a single cell has tens of...

9. Antibodies 101: Selecting the Right Antibody

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   ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...

10. Transferable Skills Guide: Problem Solving

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    ...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going...

11. Antibodies 101: Polyclonal Antibodies

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    ...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...

12. RANbodies: Reporter Nanobody Fusions

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    ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

13. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    ...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ...

14. Visualizing Protein Turnover In Situ

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    ...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids...

15. Plasmid Tools for Microbiome Studies

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    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

17. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

18. CRISPRainbow and Genome Visualization

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    ...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ...

19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post

    ...mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995...

20. Human Germline Editing Using CRISPR

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    Blog Post

    ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...