We narrowed to 583 results for: RET

1. Plasmids 101: Screens vs. Selections

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   Blog Post

   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

2. GPCRs: How Do They Work and How Do We Study Them?

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   ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

3. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

5. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

6. Human Germline Editing Using CRISPR

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   ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

7. Choosing the B(right)est Fluorescent Protein: Photostability

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   Blog Post

   ...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

8. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   Blog Post

   ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

9. Working with Nuclear Receptors

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   Blog Post

   ...signaling to peripheral effector organs, which then return information such as nutritional status. Within ...

10. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post

    ...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

11. CRISPRainbow and Genome Visualization

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    Blog Post

    ...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ...

12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post

    ...mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995...

13. The Future of Research Symposium Boston 2015

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    Blog Post

    ... Boston 2015 where we hope to begin developing concrete solutions to the problems looming over the academic...

14. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

15. Plasmid Tools for Microbiome Studies

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    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

17. Antibodies 101: Selecting the Right Antibody

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    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...

18. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going...

19. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...

20. To Each HIS Own

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    Blog Post

    ... specific proteins within a system. This can be pretty complicated given that a single cell has tens of...