Targeted Mutagenesis with EvolvR
Type
Blog Post
... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...
We narrowed to 601 results for: RET
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Screening for Successful Genome Editing with Digital PCR
TypeBlog Post..., does not require standard curves, and can theoretically detect a single target molecule in a sample.... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular... -
Human Germline Editing Using CRISPR
TypeBlog Post...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...Amsterdam) where she develops genetically encoded FRET based biosensors. Additional Resources on the... -
To Each HIS Own
TypeBlog Post... specific proteins within a system. This can be pretty complicated given that a single cell has tens of... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ... -
Visualizing Protein Turnover In Situ
TypeBlog Post...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active... -
CRISPRainbow and Genome Visualization
TypeBlog Post...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995...