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We narrowed to 749 results for: REV

Showing: 441 - 460 of 749 results
  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...conversion by engineered glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143...
  2. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post
    ... blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...considerably easier, faster, and more efficient than previous methods. Still, generating reliable results from...
  3. Transferable Skills Guide: Career Planning Resources

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    Blog Post
    ... you will not be a graduate student or postdoc forever, and you need to be thinking about what comes next... development center, if one exists. Get online! Review company and institution career pages. Make yourself...
  4. Career Insights: Technical Support Specialist

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    Blog Post
    ...takes on cases requiring in-depth analysis, data review, in-house testing etc. Technical support roles ...too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting protocols,...
  5. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...company you currently use. You can also find many previously validated gRNAs at Addgene. In vitro screening... and stuff ups). This is a huge improvement on previous mouse work, which could take 8 months or more....
  6. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID...
  7. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post
    ...seizures or some other unwanted phenomenon or it could reveal something fascinating. Behavior is based primarily...behavioral test might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral...
  8. Filming Science Videos in the Age of Social Distancing

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    Blog Post
    ...operator is in a different room.   Design: The previous cycle made me realize we needed to focus on ways...wouldn’t be in there with them. I knew from my previous cycles that I wanted to be able to talk to people...
  9. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016...
  10. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second crossover... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...
  11. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date, small probes ... tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. PubMed PMID: 28401765....
  12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...strategies. As an example, we’ll use one of my previous experiments where I harvested a melanoma tumor...single cell + live” population, acquired from the previous gating pipeline, about 53% of my cells are dendritic...
Showing: 441 - 460 of 749 results