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We narrowed to 741 results for: REV

Showing: 441 - 460 of 741 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...company you currently use. You can also find many previously validated gRNAs at Addgene. In vitro screening... and stuff ups). This is a huge improvement on previous mouse work, which could take 8 months or more....

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth...Incidence, sources, effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID...

  3. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...seizures or some other unwanted phenomenon or it could reveal something fascinating. Behavior is based primarily...behavioral test might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date, small probes ... tools engineered from phytochromes." Chemical reviews 117.9 (2017): 6423-6446. PubMed PMID: 28401765....

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second crossover... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  9. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...operator is in a different room.   Design: The previous cycle made me realize we needed to focus on ways...wouldn’t be in there with them. I knew from my previous cycles that I wanted to be able to talk to people...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...strategies. As an example, we’ll use one of my previous experiments where I harvested a melanoma tumor...single cell + live” population, acquired from the previous gating pipeline, about 53% of my cells are dendritic...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    ...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling...

  13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...expressed for a short period of time, which would prevent the accumulation of unwanted mutations over time...upregulating HDR, NHEJ must be downregulated to prevent the introduction of new mutations by CRISPR; editing...

  14. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...your success. Therefore, it only makes sense to reverse engineer your budgetary needs from your experimental...going through some of the questions we mentioned previously.  There is value in the process of creating a...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA...repair (HDR), and insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    .... Of note, most of these optimized Cas enzymes prevent cutting at mismatched guide sites, but not necessarily...alternative pathways. See (Guo et al, 2023) for a review of other targeted sequencing methods. Candidate...

  17. A Control for All Seasons

    Type
    Blog Post
    ...an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples...means an exhaustive list and we encourage you to review the literature for your specific experimental technique...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... anneal to the backbone in either a forward or reverse orientation so you’ll need some way to verify that...digestion, you will need to use a phosphatase to prevent re-circularization of the backbone plasmid (see...

  20. Important Considerations When Using AAVs

    Type
    Blog Post
    ...really does not take much time at all. Much of the previous literature using AAVs for DREADD delivery indicates... of neurons showing a very specific phenotype. Previous neural tracing methods used viruses that were ...
Showing: 441 - 460 of 741 results