We narrowed to 537 results for: SHI

1. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   .... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental...

2. CRISPRainbow and Genome Visualization

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   Blog Post

   ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

3. Plasmid Tools for Microbiome Studies

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   Blog Post

   ...: 30643213. PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic...

4. Antibodies 101: Selecting the Right Antibody

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   Blog Post

   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage...

6. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...

7. The time and cost required to make a plasmid

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   Blog Post

   ...things go wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory...

9. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate protein...

10. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...genome engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes...

11. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...

12. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    .... PubMed Central PMCID: PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based ...

13. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post

    ...attention of the audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good...

14. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    .... Since makerspaces are generally funded on a membership model - everyone contributes each month - with...

15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...

16. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...3rd generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors...

17. A Look at Addgene's QC Process

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    Blog Post

    ...the plasmid. This includes point mutations, frame shifts, missing elements, and nucleotide indels or mismatches...

18. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...Note that βME reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time...

19. Plasmids 101: Modular Cloning

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    Blog Post

    ...identify the best cocktail of genes when the relationship between genotype and phenotype is best described...

20. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ...Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow or orange-red...