We narrowed to 542 results for: SHI

1. Antibodies 101: Selecting the Right Antibody

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   Blog Post

   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

2. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage...

3. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains on either side...

4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

5. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   ...does not depend on excitation power in a linear fashion. This implies that reducing the power 2-fold does...

6. CRISPRainbow and Genome Visualization

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   Blog Post

   ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

7. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...

8. The time and cost required to make a plasmid

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   Blog Post

   ...things go wrong, replace them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up...

9. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate protein...

10. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...genome engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes...

11. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...

12. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

13. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    .... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental...

14. Working with Nuclear Receptors

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    Blog Post

    ...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the entire research...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...3rd generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors...

16. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but...

17. Hot Plasmids - October 2020

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    Blog Post

    ...available as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...

18. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ...Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow or orange-red...

19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

20. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...Sathyan V, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH,  Begeman A, Stark JC, Shon DJ, Fox DM, Puschnik...