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We narrowed to 949 results for: ache

Showing: 441 - 460 of 949 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    ...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...noting that it is possible to design a vector that achieves persistent transgene expression in certain cell...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl, H. C. J. (2007). Adenoviral...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which are summarized... your plasmid Have to make a new primer pair for each insert Backbone-Specific Provides information...cloning You have to make a new primer pair for each insert since primers are insert dependent   ...

  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... screening), or arrayed on a 96 well plate where each well contains a defined prey fusion.  The former...possible to screen libraries of bait and prey against each other at the same time in order to try and access...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, ...

  4. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ... work my lab wanted to do already seemed to know each other and what to do.  Saved by the Tweet - The...people attending large meetings. A key was to provide each bin with a mentor who had attended the meeting multiple...networking and mentoring circles We looked at a lot of approaches scientific meetings have taken to provide mentoring...Joe taught high school chemistry for a year. The teaching/mentoring bug never went away. While he has frequent...

  5. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...primers for each individual sample, this will reduce convenience and comparability because each sample with...other DNA quantification methods - see below), since each viral particle typically contains one viral genome...indication of infectious titer, consider titrating each lot of AAV in vivo to determine the optimal dosage...

  6. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...rewarding parts of the job. Solutions may also be achieved by simply replacing the product in question. This...different backgrounds and realizing that the same approach does not work for everyone. The benefit is that... in project management, consulting, scientific outreach, and more. Technical support roles also involve... images used in this post. Klaus Wanisch is an Outreach Scientist at Addgene. These days he's doing a ...

  7. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...are many types of electronic lab notebooks (ELNs), each with its own pros and cons. All ELNs have the virtue... take a look at the RSpace ELN.  The connected approach in action: how the RSpace ELN supports reproducibility...research, and data repositories. Below I describe how each of these kinds of connectivity facilitates reproducibility...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...

  8. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...required to develop new projects and test novel approaches, a necessary skill set for any scientist. In ...lab   Thinking Outside the Box: Individual Approaches Whether it’s finding the source of contamination...your situation.   Thinking Outside the Box: Team Approaches At some point or another, you’ll find yourself...benefits from their policy! Perfectionism When we reach for perfection, our work stalls. We become so fixated...

  9. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders. But CRBN...images of single focal planes through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...using standard methods that have been optimized for each specific virus in order to generate high quality... generally two to three fold higher than those achieved using the standard qPCR method.  Titering by the...The specific QC experiments performed varies for each viral lot. To learn which specific QC experiments... throughout production, ensuring the identity of each sample from the DNA tube all the way to the viral...

  11. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...are not general amplification protocols. Rather, each protocol is optimized for a single, specific library...step also depends on the individual library. So, on each library’s page, you can find protocols for sequencing...protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T cells...virus–the right amount of virus, that is. Ideally, each cell will be transduced by no more than one viral...

  12. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...salaries). Postdocs (who are not in a primarily teaching role) come under this ruling, regardless of visa...on higher education, you can see that those in teaching roles (such as adjuncts) and graduate and undergraduate...policies. It is unclear how many postdocs are at each of these institutions (populations potentially from...trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and postdoctoral...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...recombinase have been developed. A common means to achieve ligand control is to fuse a mutant estrogen receptor...be transfected by in utero electroporation. To achieve sparse labeling of individual neurons one could...the same time from two different plasmids this approach becomes problematic as it reduces the probability... the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently to DNA via a Phosphotyrosyl Linkage...

  14. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...tag. Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   References Adjobo-Hermans, Merel JW, et ...

  15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...therapeutic settings. Silencing of a target gene is achieved through the delivery of a double-stranded RNA ... plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence ...the bound miRISC can simply block translation machinery. The composition of the RISC varies, but at the... Silencing Complex: a versatile gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897...

  16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...desirable traits using traditional plant breeding approaches, these techniques are cumbersome, often requiring...highlight the specific modifications to CRISPR machinery that allow for the use of CRISPR in plants, and...in question. In plant cells, gRNA expression is achieved by placing the gRNA downstream of a plant-specific...Glycol (PEG), although the application of this approach is limited to protoplast cells (plant cells whose...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    ...allowing for the study of their function. This approach to studying DNA sequences helped researchers at...Researchers perform four different reactions, one for each radiolabelled ddNTP, and then run these reactions...generally considered first generation DNA sequencing machines (9). Today, similar technologies, sometimes referred...older technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds... using knockout or transcriptional modulation approaches. Avoiding off-target effects The off-target ...Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, ...

  19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each guide. ...When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP...strands, as well as guides facing towards or away from each other.  Figure 4: How to design a conditional...

  20. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...small number of glutamate molecules are released in each synaptic event and they are rapidly taken up by ...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer... is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger...'s important to know what proteins interact with each other and where those interactions occur in the ...
Showing: 441 - 460 of 949 results