We narrowed to 495 results for: control

...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...plate will hold 5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding...selection should last at least as long as it takes the control (untransduced) cells to completely die. After that...

...pipette 200–1000 µL single channel pipette Pipette controller Pipette tips and pipettes Spectrophotometer Heat...as expected and is specific, include a positive control sample that you know expresses the protein, such...transfected with the protein of interest, and a negative control sample that does not express the protein of interest...

...or cell type. The strength of the promoter can control the level of insert expression, as a strong promoter...of another antibiotic resistance gene under the control of a non-bacterial promoter or a fluorescent protein...that the antibiotic resistance gene is under the control of a bacterial promoter, and is thus expressed ...plasmids are sequenced with NGS during our quality control process . NGS allows us to sequence entire plasmids...

...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

... study human disease-relevant genes in such a controlled environment displays how essential the frog is...

...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR...

...2018). NIR FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools...

...use in Drosophila melanogaster and is under the control of Flp recombinase. The XFPs in Flybow are membrane-tethered...

... Living Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1...

... the strength of the signal in comparison to a control sample. Southern blotting applications range from...

...exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or absence of dNTPs. Secondly...

... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...

...express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift...µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope Hemocytometer...

...-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages...LmoCas9 and SpCas9. Acr proteins can be used to control both when and where genome editing happens. CASANOVA...Dominik Niopek’s lab , is a way to optogenetically control genome editing by fusing the anti-CRISPR protein...photosensitive LOV2 domain . Several labs have also controlled Acr activity with endogenous cell-type specific...have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA...Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLife . 3 e04766...

...deposited from this paper, a large menu of tool and control constructs is available to choose from! Find plasmids...

... Addgene as a source of sequenced and quality controlled plasmids and viral vectors. When working with...

...transcriptional regulation, as long as they can control recombinase activity. For example, post-transcriptional...

...aminoacylate the orthogonal tRNA. Therefore many controls must be used to make sure that these conditions...conditions are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...

...ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for normalization. The dilution series outlined...wells containing the virus and the untransduced control. Pro-Tip For even seeding, prepare a batch for ...concentration of RRE positive droplets in the untransduced control should be close to zero (A01). In this protocol...

...insertion of any target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert...