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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 494 results for: control

Showing: 441 - 460 of 494 results

  2. Rett Syndrome

    Type
    Collection
    ... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...plate will hold 5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding...selection should last at least as long as it takes the control (untransduced) cells to completely die. After that...

  4. Western Blot

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Pipette controller Pipette tips and pipettes Spectrophotometer Heat...as expected and is specific, include a positive control sample that you know expresses the protein, such...transfected with the protein of interest, and a negative control sample that does not express the protein of interest...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    ... the strength of the signal in comparison to a control sample. Southern blotting applications range from...

  13. Molecular Biology Reference

    Type
    Guide
    ...that the antibiotic resistance gene is under the control of a bacterial promoter, and is thus expressed ...strength of the promoter is also important for controlling the level of insert expression (i.e., a strong...antibiotic resistance gene (this time, under the control of a non-bacterial promoter) or a fluorescent protein...plasmids are sequenced with NGS during our quality control process . NGS allows us to sequence entire plasmids...

  14. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift...µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope Hemocytometer...

  16. CRISPR Guide

    Type
    Guide
    ...-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages...LmoCas9 and SpCas9. Acr proteins can be used to control both when and where genome editing happens. CASANOVA...Dominik Niopek’s lab , is a way to optogenetically control genome editing by fusing the anti-CRISPR protein...photosensitive LOV2 domain . Several labs have also controlled Acr activity with endogenous cell-type specific...have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA...Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLife . 3 e04766...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for normalization. The dilution series outlined...wells containing the virus and the untransduced control. Pro-Tip For even seeding, prepare a batch for ...concentration of RRE positive droplets in the untransduced control should be close to zero (A01). In this protocol...
Showing: 441 - 460 of 494 results