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We narrowed to 879 results for: ids

Showing: 441 - 460 of 879 results

  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    ...from researchers, and the vast majority of our plasmids and pooled libraries were originally described...links to later papers that successfully used said plasmids. We provide these references as our way of saying...Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help with the Addgene...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... genes are supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and...carrying your gene of interest, and the two helper plasmids, commonly called “triple plasmid transfection”...genome-containing (full) and non-genome-containing (empty) capsids, thus leading to an overestimate of transducible...

  5. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...fidelity and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central...

  6. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...sequencing on select regions of all distributed plasmids as part of quality control, but does not perform...complexes or with guide RNA- and Cas9-expression plasmids. Cas9 guide RNA delivery does however rely on ...happy genome editing! Check Out Other Argonaute Plasmids from the Doudna Lab Many thanks to our Guest ...

  7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ..."Addgene is one of the leaders in distributing plasmids amongst the academic community. On the BioBricks...engineering an entire cell. Typically when people use plasmids, they are trying to do one thing at a time. What... tricky to tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... the community to share their expertise with the kids through some fun activities. If your school doesn...levels, and STEM exposure. If you expect a lot of kids, you’re going to want a lot of activities (try for...out our comprehensive CRISPR guide Find CRISPR plasmids and resources ...

  10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...ribonucleoproteins? One alternative approach, which avoids many of these complications, is to directly deliver... as CMV or EF1A promoters found in many CRISPR plasmids) are not expressed. Because this method does ...design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs Check out our CRISPR guide...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...single population (e.g. transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid...the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19):e184. Pubmed PMID: 20719741. Pubmed...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

  13. Viral Production at Addgene

    Type
    Blog Post
    ...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...endA-mutated NEB Stable strain of E. coli. In addition, plasmids are typically prepared using endotoxin-free plasmid... system and PCR-based confirmation of transfer plasmids used in Addgene’s viral production. Barcoding ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...protein interacts with other proteins or nucleic acids. Read on to learn more about this versatile technique...used to study proteins that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

  16. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding nor its ability...New York. pp 719. 3.Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking...Nathan C., George H. Patterson, and Michael W. Davidson. "Advances in fluorescent protein technology."...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    ...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells (even pathogenic bacteria...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...longer than 200 bases can be difficult, thus dsDNA plasmids are generally preferred for large insertions such...edits, researchers have designed self-cleaving plasmids that liberate the targeting region from the vector... Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...
Showing: 441 - 460 of 879 results